Diameter of tumor blood vessels is a good parameter to estimate HIF-1-active regions in solid tumors.

Full Abstract

As the transcriptional activity of hypoxia-inducible factor 1 (HIF-1) is associated with resistance of tumor cells to current antitumor therapies, the spatiotemporal dynamics of HIF-1-active regions has been of great interest as a therapeutic target. In the present study, we established a unique cancer cell line, which changes color HIF-1-dependently, and monitored it during tumor progression. In imaging experiments, HIF-1-active cells appeared over the tumor xenograft, but dramatically decreased in number as blood vessels developed around the tumor. The remaining HIF-1-active cells at the center of the xenograft also disappeared after neovascularization. Thereafter, tumor growth was accelerated and HIF-1-active cells reappeared in different regions. The distance between HIF-1-active cells and the nearest vessels correlated to the diameter of the vessel (r=0.801). These results provide a basic knowledge of how to estimate the spatiotemporal dynamics of HIF-1-active cells using information about the image-guided architecture of tumor blood vessels.

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Author information

Author/s: Harada, Hiroshi (H); Xie, Xuejun (X); Itasaka, Satoshi (S); Zeng, Lihua (L); Zhu, Yuxi (Y); Morinibu, Akiyo (A); Shinomiya, Kazumi (K); Hiraoka, Masahiro (M);

Affiliation: Department of Radiation Oncology and Image-applied Therapy, Kyoto University Graduate School of Medicine, 54 Shogoin Kawahara-cho, Sakyo-ku, Kyoto 606 8507, Japan. hharada(-atsign-)kuhp.kyoto-u.ac.jp

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun), published in United States. (Language: eng)

Reference: 2008-Sep; vol 373 (issue 4) : pp 533-8

Dates: Created 2008/07/28; Completed 2008/08/25;

PMID: 18586004, status: MEDLINE (last retrieval date: 11/6/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Cell Line, Tumor
Hela Cells
Humans
Hypoxia-Inducible Factor 1 - metabolism
Luminescent Proteins - analysis

Luminescent Proteins - analysis
Mice
Neoplasm Transplantation
Neoplasms - blood supply; drug therapy
Neovascularization, Pathologic - metabolism; pathology
Xenograft Model Antitumor Assays

Associated Chemicals: Hypoxia-Inducible Factor 1 (0) ; Luminescent Proteins (0)

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