Gene expression profiling of human skeletal muscle in response to stabilized weight loss.

Full Abstract

BACKGROUND:
Diet-induced weight reduction promotes a decrease in resting energy expenditure that could partly explain the difficulty in maintaining reduced body mass. Whether this reduction remains after stabilized weight loss is still controversial, and the molecular mechanisms are unknown.

OBJECTIVE:
The objective was to investigate the effect of a stabilized 10% weight loss on body composition, metabolic profile, and skeletal muscle gene expression profiling.

DESIGN:
Obese women were assigned to a 4-wk very-low-calorie diet, a 3-6-wk low-calorie diet, and a 4-wk weight-maintenance program to achieve a 10% weight loss. Resting energy expenditure, body composition, plasma variables, and skeletal muscle transcriptome were compared before weight loss and during stabilized weight reduction.

RESULTS:
Energy restriction caused an 11% weight loss. Stabilization to the new weight was accompanied by an 11% decrease in the resting metabolic rate normalized to the body cellular mass. A large number of genes were regulated with a narrow range of regulation. The main regulated genes were slow/oxidative fiber markers, which were overexpressed, and the gene encoding the glucose metabolism inhibitor PDK4, which tended to be down-regulated. The knowledge-based approach gene set enrichment analysis showed that a set of genes related to long-term calorie restriction was up-regulated, whereas sets of genes related to insulin, interleukin 6, and ubiquitin-mediated proteolysis were down regulated.

CONCLUSIONS:
Weight loss-induced decreases in resting metabolic rate persist after weight stabilization. Changes in skeletal muscle gene expression indicate a shift toward oxidative metabolism.

Learn Faster Today Improve your study skills

Author information

Author/s: Larrouy, Dominique (D); Barbe, Pierre (P); Valle, Carine (C); Déjean, Sébastien (S); Pelloux, Véronique (V); Thalamas, Claire (C); Bastard, Jean-Philippe (JP); Le Bouil, Anne (A); Diquet, Bertrand (B); Clément, Karine (K); Langin, Dominique (D); Viguerie, Nathalie (N);

Affiliation: INSERM, U858, Obesity Research Laboratory, Institut de Médecine Moléculaire de Rangueil, Toulouse, France.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: The American journal of clinical nutrition (Am J Clin Nutr), published in United States. (Language: eng)

Reference: 2008-Jul; vol 88 (issue 1) : pp 125-32

Dates: Created 2008/07/10; Completed 2008/07/31;

PMID: 18614732, status: MEDLINE (last retrieval date: 11/6/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article (including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Absorptiometry, Photon
Adult
Basal Metabolism - genetics; physiology
Body Composition - genetics; physiology
Calorimetry, Indirect
Carbon Dioxide - analysis
Diet, Reducing
Female
Gene Expression Profiling

Humans
Middle Aged
Muscle, Skeletal - metabolism
Obesity - diet therapy; genetics
Oligonucleotide Array Sequence Analysis
Oxygen Consumption
Polymerase Chain Reaction
RNA - metabolism
Weight Loss - genetics; physiology

Associated Chemicals: Carbon Dioxide (124-38-9) ; RNA (63231-63-0)

These are the highest related articles currently in the database:

See 100+ related articles.