Research article summary:

Interactions between thrombin and natural products of Millettia speciosa Champ. using capillary zone electrophoresis.

Full Abstract

In Chinese medicine Suberect Spatholobus Stem is used to treat menoxenia, blood deficiency, numb paralyses, and so on. In folk, Millettia speciosa is often used as a substitute for Suberect Spatholobus Stem in some areas but it has not been reported whether M. speciosa is the eligible substitute for Suberect Spatholobus Stem or not till now. In this study, a capillary zone electrophoretic method was applied to determinate the interactions between natural products isolated from M. speciosa Champ. and thrombin for the first time. Both qualitative and quantitative characterizations of the molecule-enzyme binding were determined. Twenty ingredients were isolated from M. speciosa Champ. and the results showed that compared with positive and negative control, the compounds YT-1, YT-2, YT-3, YT-8, YT-9, YT-10, YT-11, YT-12, YT-14, YT-15, YT-16, and YT-20 interacted with thrombin while the other eight had no binding to thrombin. The binding constants of the interaction between compounds and thrombin were calculated by the Scatchard analysis formula. Because M. speciosa contains these compounds which have different levels of anticoagulant activity, it may be the eligible substitute for Suberect Spatholobus Stem.

This information was obtained from the public area of Pubmed, part of the National Library of Medicine (NLM). Abstract text and other information may be subject to copyright.

Free Speed Reading Course Free Learning Styles Test Test Your Learning Skills Free

Author information

First Name	LastName	Initials
	Zhang	S
	Yin	T
	Ling	X
	Liang	H
	Zhao	Y

Affiliation: Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Peking University, Beijing, P. R. China.

Grants:

Journal and publication information

Publication Type: JOURNAL ARTICLE

Journal: Electrophoresis (Electrophoresis).

Reference: 2008-Jul; vol (issue ) : pp

Language: ENG

PMID: 18633944 (status: Publisher) (last retrieval date: 7/19/2008)

External Links for this article (including full text providers, if available):

For links to sites providing the full text of this article and related topics see links. Note there may be a subscription or fee required for access to the full text.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

No MeSH Headings found.

The article may not be MeSH Indexed as yet.

These are the highest related articles currently in the database:

See 100+ related articles.