Research article summary (published 29 Sep 2009):

Behavior of rat periodontal ligament cells on fibroblast growth factor-2-immobilized titanium surfaces treated by plasma modification.

Full Abstract

The aim of this study was to investigate the behavior of rat periodontal ligament (PDL) cells cultured on fibroblast growth factor-2 (FGF-2)-immobilized titanium surfaces treated by oxygen (O2) plasma. We used cell disks (15 mm in diameter), and 35-mm culture dishes sputter-coated with titanium. These were treated with oxygen plasma and dipped in FGF-2 solution. Immobilized FGF-2 was visualized with a confocal laser-scanning microscope, and its weight was calculated to be approximately 22.6 ng/cm(2) using a quartz crystal microbalance-dissipation apparatus. The PDL cells were obtained from rat incisors. Cells from fourth subculture were seeded onto the FGF-2-immobilized titanium surface. Proliferation ratio, alkaline phosphatase (ALP) activity, and expressions of type I collagen and vascular endothelial growth factor (VEGF) mRNAs were evaluated. Proliferation ratio and expressions of type I collagen and VEGF mRNAs were significantly higher, whereas ALP activity was significantly lower in FGF-2-immobilized cells than in control group (p < 0.05). These findings suggest that oxygen plasma modification can immobilize FGF-2 onto a titanium surface. Immobilized FGF-2, although inferior to culture medium with FGF-2, influenced the proliferation of PDL cells and might have promoted collagen and vascular synthesis.

Author information

Author/s: Kokubu, Eitoyo (E); Yoshinari, Masao (M); Matsuzaka, Kenichi (K); Inoue, Takashi (T);

Affiliation: Department of Clinical Pathophysiology and Oral Health Science Center, Tokyo Dental College, Mihama-ku, Chiba 261-8501, Japan. Kokubu(-atsign-)tdc.ac.jp

Journal and publication information

Publication Type: Journal Article

Journal: Journal of biomedical materials research. Part A (J Biomed Mater Res A), published in United States. (Language: eng)

Reference: 2009-Oct; vol 91 (issue 1) : pp 69-75

Dates: Created 2009/08/26; Completed 2009/11/02;

PMID: 18767059, status: MEDLINE (last retrieval date: 11/2/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Alkaline Phosphatase - metabolism Animals Biocompatible Materials - analysis; chemistry; metabolism Cell Proliferation Cells, Cultured Collagen Type I - genetics; metabolism Dental Implants Fibroblast Growth Factor 2 - analysis; metabolism Gene Expression

Immobilized Proteins - analysis; metabolism Male Periodontal Ligament - cytology; metabolism RNA, Messenger - genetics Rats Rats, Sprague-Dawley Surface Properties Titanium - chemistry Vascular Endothelial Growth Factor A - genetics; metabolism

Associated Chemicals: Biocompatible Materials (0) ; Collagen Type I (0) ; Dental Implants (0) ; Immobilized Proteins (0) ; RNA, Messenger (0) ; Vascular Endothelial Growth Factor A (0) ; Fibroblast Growth Factor 2 (103107-01-3) ; Titanium (7440-32-6) ; Alkaline Phosphatase (EC 3.1.3.1)

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