Research article summary (published 29 Sep 2008):

Two distinct T cell subsets, CD4+ and CD8+CD60+, and their cytokines are required for in vitro induction of human ragweed-specific memory IgE responses.

Full Abstract

CD8(+)CD60(+) T cells (80-98% CD45RO(+); 20% CD23(+)) are significantly increased in the blood of serum IgE(+) ragweed-sensitized (RS) compared with serum IgE-nonatopic humans (p = 0.001). CD8(+)CD60(+) T cells of the RS patients produced IL-2, IL-4, IL-10, IL-12, IFN-alpha. and IFN-gamma, but not IL-6 or IL-13. When their PBMC were cultured with ragweed Ag (RA), peak IgE responses occurred on day 10; none was induced with non-cross-reacting or without Ag; nonatopic PBMC did not respond to any stimulant. When either CD4(+) or CD8(+)CD60(+) T cells were depleted from RS PBMC before culture with RA, no IgE responses were induced. If purified CD4(+) T cells or low numbers of CD8(+)CD60(+) T cells were added back to the depleted PBMC, IgE responses were restored. However, higher numbers of CD8(+)CD60(+) T cells totally suppressed IgE responses. Total suppression also was obtained when RS PBMC were cultured with RA and either anti-IL-2, IL-4, IL-10, IL-12, IFN-gamma (all concentrations), or IFN-alpha (low concentrations), but not anti-IL-6 or IL-13. Higher concentrations of anti-IFN-alpha potentiated IgE responses.

Author information

Author/s: Smith-Norowitz, Tamar A (TA); Silverberg, Jonathan (J); Norowitz, Kevin B (KB); Bluth, Martin H (MH); Chice, Seto (S); Joks, Rauno (R); Nowakowski, Maja (M); Durkin, Helen G (HG);

Affiliation: Center for Allergy and Asthma Research at State University of New York Downstate, State University of New York Downstate Medical Center, Brooklyn, NY 11203, USA.

Journal and publication information

Publication Type: Journal Article

Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol), published in United States. (Language: eng)

Reference: 2008-Oct; vol 181 (issue 7) : pp 4761-9

Dates: Created 2008/09/19; Completed 2008/12/24;

PMID: 18802079, status: MEDLINE (last retrieved date: 2/18/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Adult Ambrosia - immunology Antigens, CD - biosynthesis; metabolism; physiology Antigens, CD45 - biosynthesis; blood Antigens, Differentiation, T-Lymphocyte - biosynthesis; metabolism; physiology CD4-Positive T-Lymphocytes - immunology; metabolism CD8-Positive T-Lymphocytes - immunology; metabolism Cells, Cultured Cytokines - physiology Epitopes, T-Lymphocyte - biosynthesis; immunology; metabolism

Epitopes, T-Lymphocyte - biosynthesis; immunology; metabolism Female Humans Hypersensitivity, Immediate - immunology; metabolism Immunoglobulin E - biosynthesis; blood Immunologic Memory - immunology Immunosuppression Interferon-alpha - physiology Male T-Lymphocyte Subsets - immunology; metabolism

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: Antigens, CD (0) ; Antigens, Differentiation, T-Lymphocyte (0) ; CDw60 antigen (0) ; Cytokines (0) ; Epitopes, T-Lymphocyte (0) ; Interferon-alpha (0) ; Immunoglobulin E (37341-29-0) ; Antigens, CD45 (EC 3.1.3.48)

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