Research article summary (published 11 Oct 2008):

Dendritic cells release HLA-B-associated transcript-3 positive exosomes to regulate natural killer function.

Full Abstract

NKp30, a natural cytotoxicity receptor expressed on NK cells is critically involved in direct cytotoxicity against various tumor cells and directs both maturation and selective killing of dendritic cells. Recently the intracellular protein BAT3, which is involved in DNA damage induced apoptosis, was identified as a ligand for NKp30. However, the mechanisms underlying the exposure of the intracellular ligand BAT3 to surface NKp30 and its role in NK-DC cross talk remained elusive. Electron microscopy and flow cytometry demonstrate that exosomes released from 293T cells and iDCs express BAT3 on the surface and are recognized by NKp30-Ig. Overexpression and depletion of BAT3 in 293T cells directly correlates with the exosomal expression level and the activation of NK cell-mediated cytokine release. Furthermore, the NKp30-mediated NK/DC cross talk resulting either in iDC killing or maturation was BAT3-dependent. Taken together this puts forward a new model for the activation of NK cells through intracellular signals that are released via exosomes from accessory cells. The manipulation of the exosomal regulation may offer a novel strategy to induce tumor immunity or inhibit autoimmune diseases caused by NK cell-activation.

Author information

Author/s: Simhadri, Venkateswara Rao (VR); Reiners, Katrin S (KS); Hansen, Hinrich P (HP); Topolar, Daniela (D); Simhadri, Vijaya Lakshmi (VL); Nohroudi, Klaus (K); Kufer, Thomas A (TA); Engert, Andreas (A); Pogge von Strandmann, Elke (E);

Affiliation: Laboratory of Immune Therapy, Department of Internal Medicine I, Centre for Integrated Oncology Koeln Bonn, University of Cologne, Cologne, Germany. venkatsimhadri(-atsign-)googlemail.com

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: PloS one (PLoS One), published in United States. (Language: eng)

Reference: 2008-; vol 3 (issue 10) : pp e3377

Dates: Created 2008/10/14; Completed 2008/12/18; Revised 2009/06/16;

PMID: 18852879, status: MEDLINE (last retrieved date: 6/16/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Cell Line Dendritic Cells - secretion; ultrastructure Exosomes - chemistry; physiology; secretion Flow Cytometry Humans

Killer Cells, Natural - chemistry; immunology Ligands Microscopy, Electron Natural Cytotoxicity Triggering Receptor 3 - metabolism Proteins - metabolism

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: BAT3 protein, human (0) ; Ligands (0) ; NCR3 protein, human (0) ; Natural Cytotoxicity Triggering Receptor 3 (0) ; Proteins (0)

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