Research article summary (published 27 Oct 2009):

Immunolocalization of laminin and integrin in regenerating junctional epithelium of mice after gingivectomy.

Full Abstract

BACKGROUND AND OBJECTIVE: The expression patterns of adhesive proteins and extracellular matrix proteins in regenerating gingival epithelium after gingivectomy are unknown. The aim of this study was to examine the expression of laminin 1, laminin gamma(2) (a specific component of laminin 5), integrin beta(4) and integrin alpha(3) in the regenerating gingival epithelium in order to understand the mechanism of wound healing during reconstitution of the sulcular environment. MATERIAL AND METHODS: The palatal gingivae of the maxillary molars of Institute of Cancer Research mice were excised, and the regenerating tissues were examined 1, 3, 5, 7 and 14 days later. Fresh, non-fixed and non-decalcified frozen sections were prepared and stained using immunofluorescence. RESULTS: At 1 day post-surgery, intense expression of laminin gamma(2), integrin beta(4) and integrin alpha(3) was distinct in the frontal margin of the regenerating oral epithelium. Laminin gamma(2) was diffusely detected on the root surface and in connective tissues beneath the regenerating oral epithelium at 3 and 5 days. At 7 days, laminin gamma(2) was intermittently recognizable in the internal basal lamina (IBL) close to tooth-facing cells, while laminin gamma(2), integrin beta(4) and integrin alpha(3) were observed in the IBL and in the external basal lamina (EBL) of the regenerating junctional epithelium at 14 days. CONCLUSION: These results suggest that secretion of laminin 5 in the connective tissue may induce epithelial cell migration, and that binding of laminin 5 to integrin alpha(6)beta(4) and integrin alpha(3)beta(1) in the IBL may provoke cell adhesion and migration of cells facing the tooth on the enamel surface of the regenerating junctional epithelium.

Author information

Author/s: Masaoka, T (T); Hashimoto, S (S); Kinumatsu, T (T); Muramatsu, T (T); Jung, H-S (HS); Yamada, S (S); Shimono, M (M);

Affiliation: Oral Health Science Center HRC7, Tokyo Dental College, Chiba, Japan.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of periodontal research (J Periodontal Res), published in Denmark. (Language: eng)

Reference: 2009-Aug; vol 44 (issue 4) : pp 489-95

Dates: Created 2009/07/14; Completed 2009/10/29;

PMID: 18973515, status: MEDLINE (last retrieval date: 10/29/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Basement Membrane - pathology Cell Adhesion - physiology Cell Adhesion Molecules - analysis Cell Movement - physiology Connective Tissue - pathology Epithelial Attachment - pathology Epithelium - pathology Gingiva - pathology Gingivectomy Integrin alpha3 - analysis

Integrin alpha3 - analysis Integrin beta4 - analysis Integrins - analysis Laminin - analysis Male Mice Mice, Inbred ICR Regeneration - physiology Time Factors Tooth Cervix - pathology Tooth Root - pathology

Associated Chemicals: Cell Adhesion Molecules (0) ; Integrin alpha3 (0) ; Integrin beta4 (0) ; Integrins (0) ; Laminin (0) ; kalinin (0) ; laminin 1 (0)

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