Research article summary (published 29 Sep 2009):

Cocultures of osteoblasts and osteoclasts are influenced by local application of zoledronic acid incorporated in a poly(D,L-lactide) implant coating.

Full Abstract

The antiresorptive activity of bisphosphonates such as zoledronic acid (ZOL) has been shown in vitro to be because of their effect on osteoclasts and osteoblasts. However, whether the effect of ZOL on monocultures might be reproducible on cocultures and whether cell interactions might influence this effect has not been described. The aim of the study was to investigate the effect of ZOL on cocultures of osteoblasts and osteoclasts in vitro. ZOL was incorporated in an implant coating based on poly(D,L-lactide) in different concentrations (10-50 microM). Cell number was measured, and procollagen I synthesis, osteoprotegerin (OPG) secretion and soluble receptor activator of nuclear factor-kappaB ligand (sRANKL) were analyzed. Moreover, TRAP-positive cells and resorption lacunas on dentin chips were counted. Results showed that cell viability was not affected when treated with doses equivalent up to 50-microM ZOL-coated implants (ZOL-CI). Procollagen I and OPG synthesis was highest when treated with 10 microM ZOL-CI, whereas sRANKL showed no significant decrease when treated with the investigated concentrations of ZOL-CI. TRAP-positive cells were decreased when treated with ZOL-CI in a dose-dependent manner. Resorption activity of osteoclasts was not significantly decreased when treated with investigated concentrations of ZOL-CI. Exposure to specific concentrations of ZOL-CI showed a beneficial effect on osteoblast differentiation and protein synthesis. Formation of osteoclast was decreased, whereas a significant decrease in sRANKL secretion and resorption activity of osteoclasts could not be shown. The investigated effect on cocultures might be clinically useful to support fracture healing and to reduce orthopedic implant loosening.

Author information

Author/s: Greiner, S (S); Kadow-Romacker, A (A); Schmidmaier, G (G); Wildemann, B (B);

Affiliation: Center for Musculoskeletal Surgery, Julius Wolff Institut, Berlin Brandenburg Center for Regenerative Therapies, Charité-Universitätsmedizin Berlin, Augustenburger Platz 1, D-13353 Berlin, Germany. stefan.greiner(-atsign-)charite.de

Journal and publication information

Publication Type: Journal Article

Journal: Journal of biomedical materials research. Part A (J Biomed Mater Res A), published in United States. (Language: eng)

Reference: 2009-Oct; vol 91 (issue 1) : pp 288-95

Dates: Created 2009/08/26; Completed 2009/11/02;

PMID: 18980195, status: MEDLINE (last retrieval date: 11/2/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Acid Phosphatase - metabolism Bone Density Conservation Agents - administration & dosage; pharmacology Bone Resorption - metabolism Cell Count Cells, Cultured Coated Materials, Biocompatible - chemistry Coculture Techniques Collagen Type I - metabolism Diphosphonates - administration & dosage; pharmacology

Diphosphonates - administration & dosage; pharmacology Humans Imidazoles - administration & dosage; pharmacology Isoenzymes - metabolism Osteoblasts - cytology; drug effects; metabolism Osteoclasts - cytology; drug effects; metabolism Osteoprotegerin - metabolism Polyesters - chemistry RANK Ligand - metabolism

Associated Chemicals: Bone Density Conservation Agents (0) ; Coated Materials, Biocompatible (0) ; Collagen Type I (0) ; Diphosphonates (0) ; Imidazoles (0) ; Isoenzymes (0) ; Osteoprotegerin (0) ; Polyesters (0) ; RANK Ligand (0) ; collagen type I, alpha 1 chain (0) ; zoledronic acid (118072-93-8) ; poly(lactide) (26969-66-4) ; tartrate-resistant acid phosphatase (EC 3.1.3.-) ; Acid Phosphatase (EC 3.1.3.2)

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