Research article summary (published 29 Sep 2009):

Validation of a new yeast-based reporter assay consisting of human estrogen receptors alpha/beta and coactivator SRC-1: application for detection of estrogenic activity in environmental samples.

Full Abstract

Endocrine disruptors are exogenous substances that act like hormones in the endocrine system and disrupt the physiologic function of endogenous hormones. In the present study, we established reporter yeast strains (Saccharomyces cerevisiae) expressing human estrogen receptors, ERalpha or ERbeta. These strains contain a reporter plasmid carrying an estrogen responsive element (ERE) upstream of the beta-galactosidase gene, and a plasmid expressing a steroid receptor coactivator, SRC-1e. Using these reporter strains, we demonstrated dose-dependent estrogenic activities of different categories of ligands, a natural hormone, 17beta-estradiol (E2); a synthetic drug, diethylstilbestrol (DES); phytoestrogens, genistein, daizein and emodin; and an environmental endocrine disrupter, bisphenol A. EC(50) values of E2 for ERalpha and ERbeta are 5.31 x 10(-10) and 5.85 x 10(-10) M, respectively. We also demonstrated that these yeasts were applicable for measuring estrogenic activities of environmental water samples. Most downstream sites of a river showed similar activity in both ERalpha and ERbeta assays. These yeast strains are useful and convenient for detecting and comparing the estrogenic ligand activities of environmental samples in response to ERalpha and ERbeta.

Author information

Author/s: Chu, Wai-Ling (WL); Shiizaki, Kazuhiro (K); Kawanishi, Masanobu (M); Kondo, Mami (M); Yagi, Takashi (T);

Affiliation: Environmental Genetics Laboratory, Frontier Science Innovation Center and Graduate School of Science, Osaka Prefecture University, 1-2 Gakuen-cho, Naka-ku, Sakai, Osaka 599-8570 Japan.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't; Validation Studies

Journal: Environmental toxicology (Environ Toxicol), published in United States. (Language: eng)

Reference: 2009-Oct; vol 24 (issue 5) : pp 513-21

Dates: Created 2009/09/07; Completed 2009/10/09;

PMID: 19161236, status: MEDLINE (last retrieval date: 10/9/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Biological Assay Dose-Response Relationship, Drug Environmental Monitoring Estrogen Receptor alpha - metabolism Estrogen Receptor beta - metabolism Estrogens - analysis; pharmacology Genes, Reporter

Genes, Reporter Histone Acetyltransferases - metabolism Ligands Saccharomyces cerevisiae - genetics; metabolism Transcription Factors - metabolism Transcription, Genetic - drug effects Water Pollutants, Chemical - analysis; pharmacology

Associated Chemicals: Estrogen Receptor alpha (0) ; Estrogen Receptor beta (0) ; Estrogens (0) ; Ligands (0) ; Transcription Factors (0) ; Water Pollutants, Chemical (0) ; Histone Acetyltransferases (EC 2.3.1.48) ; nuclear receptor coactivator 1 (EC 2.3.1.48)

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