Research article summary (published 30 Jul 2009):

Temporal growth factor release from platelet-rich plasma, trehalose lyophilized platelets, and bone marrow aspirate and their effect on tendon and ligament gene expression.

Full Abstract

Platelet-rich plasma (PRP) has generated substantial interest for tendon and ligament regeneration because of the high concentrations of growth factors in platelet alpha-granules. This study compared the temporal release of growth factors from bone marrow aspirate (BMA), PRP, and lyophilized platelet product (PP), and measured their effects on tendon and ligament gene expression. Blood and BMA were collected and processed to yield PRP and plasma. Flexor digitorum superficialis tendon (FDS) and suspensory ligament (SL) explants were cultured in 10% plasma in DMEM (control), BMA, PRP, or PP. TGF-beta1 and PDGF-BB concentrations were determined at 0, 24, and 96 h of culture using ELISA. Quantitative RT-PCR for collagen types I and III (COL1A1, COL3A1), cartilage oligomeric matrix protein (COMP), decorin, and matrix metalloproteinases-3 and 13 (MMP-3, MMP-13) was performed. TGF-beta1 and PDGF-BB concentrations were highest in PRP and PP. Growth factor quantity was unchanged in BMA, increased in PRP, and decreased in PP over 4 days. TGF-beta1 and platelet concentrations were positively correlated. Lyophilized PP and PRP resulted in increased COL1A1:COL3A1 ratio, increased COMP, and decreased MMP-13 expression. BMA resulted in decreased COMP and increased MMP-3 and MMP-13 gene expression. Platelet concentration was positively correlated with COL1A1, ratio of COL1A1:COL3A1, and COMP, and negatively correlated with COL3A1, MMP-13, and MMP-3. White blood cell concentration was positively correlated with COL3A1, MMP3, and MMP13, and negatively correlated with a ratio of COL1A1:COL3A1, COMP, and decorin. These findings support further in vivo investigation of PRP and PP for treatment of tendonitis and desmitis. Copyright 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.

Author information

Author/s: McCarrel, Taralyn (T); Fortier, Lisa (L);

Affiliation: Department of Clinical Sciences, Cornell University, Ithaca, New York 14853, USA.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society (J Orthop Res), published in United States. (Language: eng)

Reference: 2009-Aug; vol 27 (issue 8) : pp 1033-42

Dates: Created 2009/07/27; Completed 2009/08/18;

PMID: 19170097, status: MEDLINE (last retrieval date: 8/21/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Blood Platelets - drug effects; physiology Bone Marrow - physiology Collagen Type I - biosynthesis Collagen Type III - biosynthesis Gene Expression - drug effects Horses Intercellular Signaling Peptides and Proteins - secretion Ligaments - metabolism

Ligaments - metabolism Matrix Metalloproteinase 13 - biosynthesis Matrix Metalloproteinase 3 - biosynthesis Platelet-Derived Growth Factor - biosynthesis Platelet-Rich Plasma - metabolism RNA, Messenger - metabolism Tendons - metabolism Transforming Growth Factor beta1 - biosynthesis Trehalose - pharmacology

Associated Chemicals: Collagen Type I (0) ; Collagen Type III (0) ; Intercellular Signaling Peptides and Proteins (0) ; Platelet-Derived Growth Factor (0) ; RNA, Messenger (0) ; Transforming Growth Factor beta1 (0) ; platelet-derived growth factor BB (0) ; Trehalose (99-20-7) ; Matrix Metalloproteinase 13 (EC 3.4.24.-) ; Matrix Metalloproteinase 3 (EC 3.4.24.17)

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