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| Research article summary (published 25 Jan 2009): |
Paclitaxel production using co-culture of Taxus suspension cells and paclitaxel-producing endophytic fungi in a co-bioreactor.
Full Abstract
The co-culture of the suspension cells of Taxus chinensis var. mairei and its endophytic fungi, Fusarium mairei, in a 20-L co-bioreactor was successfully established for paclitaxel production. The co-bioreactor consists of two-unit tanks (10 L each) with a repairable separate membrane in the center, culturing Taxus suspension cells in one tank and growing fungi in another. By optimizing the co-culture conditions, there was a desirable yield of paclitaxel in Taxus cell cultures. The Taxus cell cultures by co-culture produced 25.63 mg/L of paclitaxel within 15 days; it was equivalent to a productivity of 1.71 mg/L per day and 38-fold higher than that by uncoupled culture (0.68 mg/L within 15 days). The optimum conditions for co-culture in the co-bioreactor were: B5 medium, inoculating fungi when Taxus cells had grown for 5 days in the co-bioreactor, hydrophilic separate membrane in the center of the co-bioreactor, and air flow rate of 1:0.85 v/v/m in fungus cultures.
Author information
Author/s: Li, Yong-Cheng (YC); Tao, Wen-Yi (WY); Cheng, Long (L);
Affiliation: Key Laboratory of Industrial Biotechnology of the Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, People's Republic of China.
Journal and publication information
Publication Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't
Journal: Applied microbiology and biotechnology (Appl Microbiol Biotechnol), published in Germany. (Language: eng)
Reference: 2009-May; vol 83 (issue 2) : pp 233-9
Dates: Created 2009/05/04; Completed 2009/05/26;
PMID: 19172266, status: MEDLINE (last retrieval date: 5/26/2009, IMS Date: )
Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.
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