Research article summary (published 30 May 2009):

Identification of hydrogen peroxide as a major cytotoxic component in Maillard reaction mixtures and coffee.

Full Abstract

The cytotoxic activity of Maillard reaction products and coffee was studied using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay and the neutral red uptake (NRU) assay. Equimolar mixtures of sugars and lysine were heated at 120 degrees C and used to stimulate bovine aorta endothelial cells for 24 h. The cytotoxic activity increased with increase in educt concentration and heating time. Mixtures containing ribose were most active, followed by lactose and glucose. Hydrogen peroxide, which was present in the Maillard mixtures in concentrations between 7 and 87 microM, was identified as one of their major cytotoxic components. H2O2-concentrations increased further up to 130 microM under cell culture conditions. Filter coffee, espresso, and green coffee extract reduced cell viability significantly to 10, 19, and 83% of PBS-treated control. The effect was largely attenuated by the addition of catalase. Nil, 33, and 41 microM H2O2 was measured in green coffee extract, filter coffee, and espresso, respectively, increasing to 13, 369, and 333 microM during cell culture conditions. No additional H2O2 formation was detected when coffee was incubated for up to 5 h without further treatment. In conclusion, hydrogen peroxide is a major product in Maillard mixtures and coffee inducing cell death in vitro.

Author information

Author/s: Hegele, Jörg (J); Münch, Gerald (G); Pischetsrieder, Monika (M);

Affiliation: Department of Chemistry and Pharmacy, Food Chemistry, Friedrich-Alexander University Erlangen, Germany.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Molecular nutrition & food research (Mol Nutr Food Res), published in Germany. (Language: eng)

Reference: 2009-Jun; vol 53 (issue 6) : pp 760-9

Dates: Created 2009/06/17; Completed 2009/08/26;

PMID: 19199286, status: MEDLINE (last retrieval date: 8/26/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Cattle Cell Survival - drug effects Cells, Cultured Coffee - chemistry Formazans - metabolism

Formazans - metabolism Hydrogen Peroxide - pharmacology Hydrogen-Ion Concentration Maillard Reaction Neutral Red - pharmacokinetics Tetrazolium Salts - metabolism

Associated Chemicals: Coffee (0) ; Formazans (0) ; Tetrazolium Salts (0) ; MTT formazan (23305-68-2) ; Neutral Red (553-24-2) ; Hydrogen Peroxide (7722-84-1)

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