Human periodontal cells initiate mineral-like nodules in vitro.

Full Abstract

A primary objective in the treatment of periodontal diseases is the regeneration of the mineralized and soft connective tissue components of the attachment apparatus. Current theories suggest the cells of the periodontium have the capacity, when appropriately triggered, to actively participate in restoring connective tissues, including mineralized tissues. To evaluate further the role of such cells in periodontal homeostasis, periodontal ligament (PDL) cells and gingival fibroblasts (GF) were cultured and examined for alkaline phosphatase levels and for the ability to produce mineralized nodules in culture. These are two characteristics of osteoblast-like cells in vitro. The levels of alkaline phosphatase produced by these cells were determined by a modified kinetic assay and the ability of these cells to produce mineral-like nodules in vitro was evaluated by Von Kossa staining and light and electron microscopy. PDL cells had significantly higher levels of alkaline phosphatase when compared with gingival fibroblasts obtained from the same patient and the same passage, in vitro. Furthermore PDL cells, but not GF, were capable of producing mineral-like nodules in vitro. These results indicate differences in behavior between PDL cells and GF; such differences may prove important in designing appropriate clinical therapies directed at stimulating periodontal regeneration.

Author information

Author/s: Arceo, N (N); Sauk, J J (JJ); Moehring, J (J); Foster, R A (RA); Somerman, M J (MJ);

Affiliation: Department of Periodontics, University of Maryland Dental School, Baltimore.

Grants: DE-08648 (Agency:NIDCR NIH HHS) ; DE-09532 (Agency:NIDCR NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, U.S. Gov't, P.H.S.

Journal: Journal of periodontology (J Periodontol), published in UNITED STATES. (Language: eng)

Reference: 1991-Aug; vol 62 (issue 8) : pp 499-503

Dates: Created 1991/11/21; Completed 1991/11/21; Revised 2007/11/14;

PMID: 1920017, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

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