 |
| Research article summary (published 27 Feb 2009): |
Recapitulation of zebrafish sncga expression pattern and labeling the habenular complex in transgenic zebrafish using green fluorescent protein reporter gene.
Full Abstract
Human synuclein family consists of alpha-, beta-, and gamma-synucleins. Here, we cloned three genes, sncb, sncga and sncgb from zebrafish. They encode beta-, gamma1-, and gamma2-synucleins, respectively. The zSyn-beta, zSyn-gamma1, and zSyn-gamma2 proteins display 69%, 47%, and 50% identity to human beta-synuclein and gamma-synuclein, respectively. By reverse transcriptase-polymerase chain reaction, we demonstrated that sncb and sncga mRNA were abundant in brain and eye, while sncgb expression was moderate in brain, kidney, ovary and testis. The 1.8-kb 5'-upstream/promoter region of the sncga gene was sufficient to direct green fluorescent protein (GFP) expression in the central nervous system and cranial ganglions. A transgenic line, Tg(sncga:GFP), was generated and its GFP expression is similar to that of endogenous sncga mRNA. Moreover, this line also labels the habenular complex and the domain of GFP expression is larger in the left than in the right habenula. Thus, this line can be used to study sncga gene regulation and for left-right asymmetry study in zebrafish brain. (c) 2009 Wiley-Liss, Inc.
Author information
Author/s: Chen, Yi-Chung (YC); Cheng, Chia-Hsiung (CH); Chen, Gen-Der (GD); Hung, Chin-Chun (CC); Yang, Chung-Hsiang (CH); Hwang, Sheng-Ping L (SP); Kawakami, Koichi (K); Wu, Bo-Kai (BK); Huang, Chang-Jen (CJ);
Affiliation: Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan.
Journal and publication information
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal: Developmental dynamics : an official publication of the American Association of Anatomists (Dev Dyn), published in United States. (Language: eng)
Reference: 2009-Mar; vol 238 (issue 3) : pp 746-54
Dates: Created 2009/03/03; Completed 2009/05/04;
PMID: 19235732, status: MEDLINE (last retrieval date: 5/4/2009, IMS Date: )
Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.
External Links for this article
(including full text providers, if available):
Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.
This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.
MeSH headings (categories)
This article was linked to the MESH Headings shown below.
Related articles
This article has not been indexed for related articles as yet, however you can still use the live related article search links below.
See a large map of 100+ related articles.