Research article summary (published 21 Feb 2009):

An endophytic taxol-producing fungus from Taxus x media, Aspergillus candidus MD3.

Full Abstract

An endophytic taxol-producing fungus (strain MD3) isolated from the inner bark of Taxus x media was identified as Aspergillus candidus according to its morphological characteristics, physiological and biochemical characteristics, and 18S rRNA gene sequence analysis. Taxol produced by A. candidus MD3 was shown to be identical to authentic taxol analyzed by UV, HPLC, MS and nuclear magnetic resonance spectroscopy. The gene encoding the 10-deacetylbaccatin III-10-O-acetyl transferase, which catalyzes formation of the last diterpene intermediate in the taxol biosynthetic pathway, has been cloned from A. candidus MD3 for the first time and possesses high homology to the same gene found in Taxus spp.

Author information

Author/s: Zhang, Peng (P); Zhou, Peng-Peng (PP); Yu, Long-Jiang (LJ);

Affiliation: Key Laboratory of Molecular Biophysics Ministry of Education, Department of Biotechnology, Institute of Resource Biology and Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: FEMS microbiology letters (FEMS Microbiol Lett), published in England. (Language: eng)

Reference: 2009-Apr; vol 293 (issue 2) : pp 155-9

Dates: Created 2009/06/15; Completed 2009/08/11;

PMID: 19239498, status: MEDLINE (last retrieval date: 8/21/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Acetyltransferases - genetics Aspergillus - classification; genetics; isolation & purification; metabolism Chromatography, High Pressure Liquid Cloning, Molecular DNA, Fungal - chemistry; genetics DNA, Ribosomal - chemistry; genetics Fungal Proteins - genetics Genes, rRNA Magnetic Resonance Spectroscopy

Mass Spectrometry Molecular Sequence Data Paclitaxel - chemistry; metabolism Plant Bark - microbiology RNA, Fungal - genetics RNA, Ribosomal, 18S - genetics Sequence Analysis, DNA Spectrophotometry, Ultraviolet Taxus - microbiology

Associated Chemicals: DNA, Fungal (0) ; DNA, Ribosomal (0) ; Fungal Proteins (0) ; RNA, Fungal (0) ; RNA, Ribosomal, 18S (0) ; Paclitaxel (33069-62-4) ; Acetyltransferases (EC 2.3.1.-)

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