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| Research article summary (published 2 Mar 2009): |
Generation of high-yield rapamycin-producing strains through protoplasts-related techniques.
Full Abstract
Rapamycin is a 31-member ring macrolide produced by Streptomyces hygroscopicus and has many applications in clinical medicine. In the present work, several protoplasts-related techniques including protoplasts mutation, intraspecies and interspecies protoplasts fusion were tried to improve the rapamycin productivity in S. hygroscopicus. Although mutation and fusion of different protoplasts of S. hygroscopicus did not improve the productivity of rapamycin significantly, the interspecies fusion of protoplasts of S. hygroscopicus D7-804 and Streptomyces erythreus ZJU325 could have brought about one high-yield (345 mg/L) rapamycin producer with 23.6% higher than that of the parental strain. Then, with seven mutants of S. hygroscopicus with different features and rapamycin productivities as the parental strains, only one-round genome shuffling has generated a high-yield rapamycin-producing strain with an outstanding yield of 445 mg/L. The systematic research of protoplast-related techniques has established an applicable way to generate high-yield strains from original microorganisms which can only produce low amount of expected natural products, without information of target gene clusters and gene sequences.
Author information
Author/s: Chen, Xiyang (X); Wei, Peilian (P); Fan, Limei (L); Yang, Dong (D); Zhu, Xiangchen (X); Shen, Wenhe (W); Xu, Zhinan (Z); Cen, Peilin (P);
Affiliation: Institute of Bioengineering, Zhejiang University, Hangzhou 310027, China.
Journal and publication information
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal: Applied microbiology and biotechnology (Appl Microbiol Biotechnol), published in Germany. (Language: eng)
Reference: 2009-Jun; vol 83 (issue 3) : pp 507-12
Dates: Created 2009/05/22; Completed 2009/06/09;
PMID: 19259658, status: MEDLINE (last retrieved date: 6/9/2009)
Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.
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