Effect of seven tricyclic diterpenoids from needles of Taxus media var. Hicksii on stimulus-induced superoxide generation, tyrosyl or serine/threonine phosphorylation and translocation of cytosolic compounds to the cell membrane in human neutrophils.

Full Abstract

Taxol has been widely used as an anticancer drug for ovarian, breast, lung and prostate cancer. Some kinds of Taxus plants are widely distributed in the Northeast Asia region. We have isolated seven tricyclic diterpenoids, taxinine, taxagifine, 5-O-cinnamoyltaxacin I triacetate, 5-decinnamoyltaxinine J, 5-cinnamoyl-9-acetyltaxicin I, taxacin and taxol from the needles of Taxus media var. Hicksii, and investigated their effects on stimulus-induced superoxide generation and translocation of cytosolic compounds to the cell membrane in human neutrophils. Six tricyclic diterpenoids used in this experiment suppressed the superoxide generation induced by N-formyl-methionyl-leucyl-phenylalanine (fMLP) and arachidonic acid (AA) in a concentration-dependent manner. Taxinine significantly suppressed the superoxide generation induced by phorbol 12-myristate 13-acetate (PMA). The compounds also suppressed fMLP- and AA-induced tyrosyl or PMA-induced serine/threonine phosphorylation, and translocation of cytosolic compounds, p47 (phox), p67 (phox) and Rac to the cell membrane in parallel with the suppression of the stimulus-induced superoxide generation.

Author information

Author/s: Tong, Jia (J); Lu, Jincai (J); Zhang, Na (N); Chi, Haidong (H); Yamashita, Koichi (K); Manabe, Masanobu (M); Kodama, Hiroyuki (H);

Affiliation: Department of Anesthesiology and Critical Care Medicine, Kochi Medical School, Nankoku Shi, Kochi, Japan.

Journal and publication information

Publication Type: Journal Article

Journal: Planta medica (Planta Med), published in Germany. (Language: eng)

Reference: 2009-Jun; vol 75 (issue 8) : pp 814-22

Dates: Created 2009/06/23; Completed 2009/10/05;

PMID: 19288401, status: MEDLINE (last retrieval date: 10/5/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Amino Acids - metabolism
Antineoplastic Agents, Phytogenic - isolation & purification; pharmacology
Biological Transport - drug effects
Cell Membrane - drug effects
Cells, Cultured
Cytosol - drug effects
Diterpenes - isolation & purification; pharmacology
Humans
NADPH Oxidase - metabolism
Neutrophils - drug effects

Neutrophils - drug effects
Oxidants - pharmacology
Phosphoproteins - metabolism
Phosphorylation - drug effects
Plant Extracts - isolation & purification; pharmacology
Plant Leaves
Superoxides - metabolism
Taxoids - isolation & purification; pharmacology
Taxus - chemistry
rac GTP-Binding Proteins - metabolism

Associated Chemicals: Amino Acids (0) ; Antineoplastic Agents, Phytogenic (0) ; Diterpenes (0) ; Oxidants (0) ; Phosphoproteins (0) ; Plant Extracts (0) ; Taxoids (0) ; neutrophil cytosol factor 67K (0) ; Superoxides (11062-77-4) ; NADPH Oxidase (EC 1.6.3.1) ; neutrophil cytosolic factor 1 (EC 1.6.3.1) ; rac GTP-Binding Proteins (EC 3.6.5.2)

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