Research article summary (published 15 Mar 2009):

Involvement of matrix metalloproteinases on the inhibition of cells invasion and migration by emodin in human neuroblastoma SH-SY5Y cells.

Full Abstract

Emodin (1,3,8-trihydroxy-6-methylanthaquinone), an active component present in the root and rhizome of Rheum palmatum L. (Polygonaceae) has anti-bacterial, anti-tumor, diuretic and vasorelaxant effects. However, its mechanism of action on the cell migration and invasion of human neuroblastoma cancer SH-SY5Y cells is not fully understood. In this study, firstly, the effects of emodin on the percentage of viable cells were examined by using MTT assay and it was found that emodin induced dose-and time-dependent inhibition in human neuroblastoma SH-SY5Y cells. Second, the effects of emodin on the migration and invasion of SH-SY5Y cells were examined by using wound assay and matrigel counting and the results showed that emodin suppressed the migration and invasion of SH-SY5Y cells. Third, we examined the effect of emodin on the levels of associated proteins by using Western blotting and the results indicated that emodin inhibited the levels of GRB2, RhoA, HIF-1alpha, VEGF, FAK, iNOS, COX2, p-p38, p-c-jun, MMP2, MMP9 and MMP7 but promoted the levels of PKC, PI3K, MEKK3 and NF-kappaB p65 that led to the inhibition of migration and invasion of SH-SY5Y cells in vitro.

Author information

Author/s: Lu, Hsu-Feng (HF); Lai, Kuang-Chi (KC); Hsu, Shu-Chun (SC); Lin, Hui-Ju (HJ); Kuo, Chao-Lin (CL); Liao, Ching-Lung (CL); Yang, Jai-Sing (JS); Chung, Jing-Gung (JG);

Affiliation: Department of Clinical Pathology, Cheng Hsin Rehabilitation Medical Center, Taipei, Taiwan, ROC.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Neurochemical research (Neurochem Res), published in United States. (Language: eng)

Reference: 2009-Sep; vol 34 (issue 9) : pp 1575-83

Dates: Created 2009/07/23; Completed 2009/10/19;

PMID: 19291397, status: MEDLINE (last retrieval date: 10/19/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Cell Line, Tumor Cell Movement - drug effects Cell Survival - drug effects Emodin - pharmacology Focal Adhesion Kinase 1 - metabolism Humans Matrix Metalloproteinase 2 - metabolism

Matrix Metalloproteinase 2 - metabolism Matrix Metalloproteinase 7 - metabolism Matrix Metalloproteinase 9 - metabolism Matrix Metalloproteinases - physiology Neoplasm Invasiveness - prevention & control Neuroblastoma - pathology Wound Healing - drug effects

Associated Chemicals: Emodin (518-82-1) ; Focal Adhesion Kinase 1 (EC 2.7.1.112) ; PTK2 protein, human (EC 2.7.1.112) ; Matrix Metalloproteinases (EC 3.4.24.-) ; Matrix Metalloproteinase 7 (EC 3.4.24.23) ; Matrix Metalloproteinase 2 (EC 3.4.24.24) ; Matrix Metalloproteinase 9 (EC 3.4.24.35)

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