Measurement of phosphorylated extracellular signal-regulated kinase 1 and 2 in an undergraduate teaching laboratory with ALPHAscreen technology.

Full Abstract

Teaching the practical aspects of signal transduction to large undergraduate classes can be challenging when there is only a finite time frame in which to engage in laboratory activities. This teaching resource describes the use of bead-based ALPHAscreen technology for a class of 300 second-year biochemistry students, exposing the next generation of researchers to cutting-edge technology. Although in this case phosphorylated extracellular signal-regulated kinase 1 and 2 were measured, this technology is applicable to the measurement of many different signaling components. This resource provides a practical guide for instructors and exemplifies how such traditionally high-throughput research technologies can be used as teaching tools.

Author information

Author/s: Hay, Debbie L (DL);

Affiliation: University of Auckland, New Zealand. dl.hay(-atsign-)auckland.ac.nz

Journal and publication information

Publication Type: Journal Article

Journal: Science signaling (Sci Signal), published in United States. (Language: eng)

Reference: 2009-; vol 2 (issue 62) : pp tr1

Dates: Created 2009/03/18; Completed 2009/06/01;

PMID: 19293430, status: MEDLINE (last retrieval date: 6/1/2009, IMS Date: 01 Jun 2009 00:00:00)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Biochemistry - education
Calcitonin
Education, Medical, Undergraduate - methods
Laboratory Techniques and Procedures
Microspheres

Mitogen-Activated Protein Kinase 1 - analysis; metabolism
Mitogen-Activated Protein Kinase 3 - analysis; metabolism
Phosphorylation
Signal Transduction - physiology
Teaching Materials

Associated Chemicals: Calcitonin (9007-12-9) ; Mitogen-Activated Protein Kinase 1 (EC 2.7.1.37) ; Mitogen-Activated Protein Kinase 3 (EC 2.7.1.37)

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