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| Research article summary (published 15 Apr 2009): |
Biomolecular oxidative damage activated by enzymatic logic systems: biologically inspired approach.
Full Abstract
Systems that perform oxidative damage to biomolecules through catalytic cascades in the presence of iron-redox labile species were activated by enzymatic logic gates that process chemical input signals according to built-in logic operations. AND/OR enzymatic logic gates were composed of glucose oxidase (GOx) and GOx/esterase, respectively. The AND/OR logic functions of the enzyme gates were activated by application of glucose-oxygen and glucose-ethyl acetate input signals, respectively. The enzymatic logic gates, upon activation by specific patterns of the chemical input signals, produced acidic solutions and triggered release of redox labile iron species from a complex that is unstable under acidic conditions. This resulted in the activation of a catalytic cascade, which produced reactive oxygen species (ROS) and subsequently yielded oxidative damage in biomolecules. Functional integration of the enzyme-based logic systems with the catalytic redox cascade that performs damage in biomolecules on demand is a first step towards "smart" systems capable of programmed detection, identification, and neutralization of potential biohazards.
Author information
Author/s: Zhou, Jian (J); Melman, Galina (G); Pita, Marcos (M); Ornatska, Maryna (M); Wang, Xuemei (X); Melman, Artem (A); Katz, Evgeny (E);
Affiliation: Department of Chemistry and Biomolecular Science and NanoBio Laboratory, Clarkson University, Potsdam, NY 13699-5810, USA.
Journal and publication information
Publication Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
Journal: Chembiochem : a European journal of chemical biology (Chembiochem), published in Germany. (Language: eng)
Reference: 2009-Apr; vol 10 (issue 6) : pp 1084-90
Dates: Created 2009/04/13; Completed 2009/06/04;
PMID: 19308926, status: MEDLINE (last retrieval date: 6/4/2009, IMS Date: 04 Jun 2009 00:00:00)
Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.
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