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| Research article summary (published 29 Apr 2009): |
Identification of taxanes in extracts from leaves of Taxus baccata L. using (13)C-NMR spectroscopy.
Full Abstract
INTRODUCTION: The study of taxanes is a hot topic worldwide because of their potential activity against cancer. Analysis of taxanes in extracts of various Taxus species is generally achieved using HPLC. OBJECTIVE: The aim of the current work was to identify taxanes in different fractions of extracts from Taxus baccata L. using the computer-aided analysis of their( 13)C-NMR spectra. METHODOLOGY: A computerised procedure, based on (13)C-NMR spectroscopy using literature data, has been developed in order to identify taxoids in natural mixtures. Leaves and twigs of T. baccata were extracted with various solvents and the extracts were fractionated according to rapid fractionation schemes described in the literature. RESULTS: The computer-assisted analysis of the (13)C-NMR spectra of the fractions of chromatography led to the identification of 16 taxanes belonging to the taxine, taxicine, taxinine and taxuspine families. CONCLUSION: It has been shown that (13)C-NMR is a powerful alternative tool for the identification of taxanes in extracts of leaves and twigs of various Taxus species after a rapid fractionation step. Copyright (c) 2009 John Wiley & Sons, Ltd.
Author information
Author/s: Duquesnoy, Emilie (E); Paoli, Mathieu (M); Castola, Vincent (V); Bighelli, Ange (A); Casanova, Joseph (J);
Affiliation: Université de Corse-CNRS, UMR 6134 SPE, Equipe Chimie et Biomasse, Route des Iles Sanguinaires, 20000 Ajaccio, France.
Journal and publication information
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal: Phytochemical analysis : PCA (Phytochem Anal), published in England. (Language: eng)
Reference: 2009-May; vol 20 (issue 3) : pp 246-52
Dates: Created 2009/04/14; Completed 2009/06/12;
PMID: 19333914, status: MEDLINE (last retrieval date: 6/12/2009, IMS Date: )
Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.
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