Research article summary (published 29 Sep 2009):

Histological and molecular analysis of the biceps tendon long head post-tenotomy.

Full Abstract

Tendinopathy is a vexing clinical problem as its onset and development is often asymptomatic and unrecognized until tendon rupture. While extensively studied in the rotator cuff, Achilles, and patellar tendons, no study to date has examined the histological and molecular characteristics of the tendinopathic biceps long-head (LHB). The anatomy of the LHB is unique in that it comprises intra- and extra-articular portions, each exposed to differing loading patterns. Eleven LHBs post-tenotomy were sectioned, fixed in formalin, and stained (H and E; Alcian Blue), and gross structural organization of collagen measured using polarized light microscopy. Protein expression of intra- and extra-articular portions of the tenotomized biceps for IGF-I, collagen III, and MMP-1, -2, -3, and -13 was determined with Western blot analyses. The intra-articular LHB exhibited significantly greater histological evidence of tendinopathy inclusive of increased proteoglycan (p < 0.05) and decreased organization as measured by polarized light microscopy (p < 0.01). The intra-articular LHB also had significantly increased expression of collagen type III (p < 0.01) and of MMP-1 and 3 (p < 0.01, p < 0.05 respectively). No significant differences were found for IGF-I or for MMP-2 and -13. The intra-articular LHB exhibited histological characteristics of tendinopathy. Protein expression of the intra-articular LHB did not universally display signs of tendinopathy in comparison to the extra-articular portion of the tendon.

Author information

Author/s: Joseph, Michael (M); Maresh, Carl M (CM); McCarthy, Mary Beth (MB); Kraemer, William J (WJ); Ledgard, Felicia (F); Arciero, Cristina L (CL); Anderson, Jeffrey M (JM); Nindl, Bradley C (BC); Mazzocca, Augustus D (AD);

Affiliation: Department of Kinesiology, The University of Connecticut, Storrs, Connecticut, USA. mickpt(-atsign-)sbcglobal.net

Journal and publication information

Publication Type: Journal Article

Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society (J Orthop Res), published in United States. (Language: eng)

Reference: 2009-Oct; vol 27 (issue 10) : pp 1379-85

Dates: Created 2009/09/21; Completed 2009/10/08;

PMID: 19340876, status: MEDLINE (last retrieval date: 10/8/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adult Arm Collagen Type III - metabolism Humans Insulin-Like Growth Factor I - metabolism Male Matrix Metalloproteinase 1 - metabolism

Matrix Metalloproteinase 13 - metabolism Matrix Metalloproteinase 2 - metabolism Matrix Metalloproteinase 3 - metabolism Middle Aged Muscle, Skeletal Shoulder Joint Tendons - metabolism; pathology; surgery

Associated Chemicals: Collagen Type III (0) ; Insulin-Like Growth Factor I (67763-96-6) ; Matrix Metalloproteinase 13 (EC 3.4.24.-) ; Matrix Metalloproteinase 3 (EC 3.4.24.17) ; Matrix Metalloproteinase 2 (EC 3.4.24.24) ; Matrix Metalloproteinase 1 (EC 3.4.24.7)

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