Mesenchymal stem cells and insulin-like growth factor-I gene-enhanced mesenchymal stem cells improve structural aspects of healing in equine flexor digitorum superficialis tendons.

Full Abstract

Tendinitis remains a catastrophic injury among athletes. Mesenchymal stem cells (MSCs) have recently been investigated for use in the treatment of tendinitis. Previous work has demonstrated the value of insulin-like growth factor-I (IGF-I) to stimulate cellular proliferation and tendon fiber deposition in the core lesion of tendinitis. This study examined the effects of MSCs, as well as IGF-I gene-enhanced MSCs (AdIGF-MSCs) on tendon healing in vivo. Collagenase-induced bilateral tendinitis lesions were created in equine flexor digitorum superficialis tendons (SDFT). Tendons were treated with 10 x 10(6) MSCs or 10 x 10(6) AdIGF-MSCs. Control limbs were injected with 1 mL of phosphate-buffered saline (PBS). Ultrasound examinations were performed at t = 0, 2, 4, 6, and 8 weeks. Horses were euthanized at 8 weeks and SDFTs were mechanically tested to failure and evaluated for biochemical composition and histologic characteristics. Expression of collagen types I and III, IGF-I, cartilage oligomeric matrix protein (COMP), matrix metalloproteinase-3 (MMP-3), matrix metalloproteinase-13 (MMP-13), and aggrecanase-1 (ADAMTS-4) were similar in MSC and control tendons. Both MSC and AdIGF-MSC injection resulted in significantly improved tendon histological scores. These findings indicate a benefit to the use of MSCs and AdIGF-MSCs for the treatment of tendinitis. (c) 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.

Author information

Author/s: Schnabel, Lauren V (LV); Lynch, Maureen E (ME); van der Meulen, Marjolein C H (MC); Yeager, Amy E (AE); Kornatowski, Matthew A (MA); Nixon, Alan J (AJ);

Affiliation: Comparative Orthopaedics Laboratory, Department of Clinical Sciences, C3-187 Veterinary Medical Center, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society (J Orthop Res), published in United States. (Language: eng)

Reference: 2009-Oct; vol 27 (issue 10) : pp 1392-8

Dates: Created 2009/09/21; Completed 2009/10/08;

PMID: 19350658, status: MEDLINE (last retrieval date: 10/8/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

ADAM Proteins - metabolism
Animals
Biomechanics
Collagen Type I - metabolism
Collagen Type III - metabolism
Extracellular Matrix Proteins - metabolism
Female
Gene Therapy - methods; veterinary
Glycoproteins - metabolism
Horse Diseases - metabolism; pathology; therapy
Horses

Horses
Insulin-Like Growth Factor I - genetics; metabolism
Male
Matrix Metalloproteinase 13 - metabolism
Matrix Metalloproteinase 3 - metabolism
Mesenchymal Stem Cell Transplantation - methods; veterinary
Mesenchymal Stem Cells - cytology; metabolism
Procollagen N-Endopeptidase - metabolism
Tendinopathy - metabolism; therapy; veterinary
Tendons - metabolism; pathology; ultrasonography
Tissue Therapy - methods; veterinary

Associated Chemicals: Collagen Type I (0) ; Collagen Type III (0) ; Extracellular Matrix Proteins (0) ; Glycoproteins (0) ; cartilage matrix protein (0) ; Insulin-Like Growth Factor I (67763-96-6) ; ADAM Proteins (EC 3.4.24.-) ; Matrix Metalloproteinase 13 (EC 3.4.24.-) ; aggrecanase-1 (EC 3.4.24.-) ; Procollagen N-Endopeptidase (EC 3.4.24.14) ; Matrix Metalloproteinase 3 (EC 3.4.24.17)

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