Research article summary (published 7 Apr 2009):

The 5-HT2A receptor is mainly expressed in nociceptive sensory neurons in rat lumbar dorsal root ganglia.

Full Abstract

Several lines of evidence indicate that peripheral 5-HT2A receptors are involved in the development of inflammatory and neuropathic pain. However, their localization in sensory cell bodies is not accurately known. We therefore studied 5-HT2A receptor distribution in rat lumbar dorsal root ganglia using immunocytochemistry. Forty percent of L3 lumbar dorsal root ganglion cells were immunoreactive for 5-HT2A receptor. Most were small- to medium-sized cell bodies. Double-labeled experiments revealed that they expressed various chemical phenotypes. The smaller 5-HT2AR cell bodies often bind the isolectin B4 although some 5-HT2AR cell bodies also express substance P (SP). Many 5-HT2A-positive small dorsal root ganglion cells expressed the capsaicin receptor transient receptor potential vanilloid type 1 receptor (TRPV1), confirming their nociceptive nature. In addition, a few large cell bodies were labeled for 5-HT2A, and they also expressed NF200 suggesting that they were at the origin of Adelta or Abeta fibers. A total absence of double labeling with parvalbumin showed that they were not proprioceptors. 5-HT2A immunoreactivity in dorsal root ganglia cells was found in the cytoplasm and along the plasma membrane at the interface between sensory cell and the adjacent satellite cells; this distribution was confirmed under the electron microscope, and suggested a functional role for the 5-HT2A receptor at these sites. We therefore investigated the presence of 5-HT and 5-HIAA in lumbar dorsal root ganglia by high performance liquid chromatography. There were 5.75+/-0.80 ng 5-HT and 3.19+/-0.37 ng 5-hydroxyindoleacetic acid (5-HIAA) per mg of protein with a ratio 5-HIAA/5-HT of 0.67+/-0.10, similar to values typically observed in brain tissues. These findings suggest that 5-HT, via the 5-HT2AR, may be involved in the peripheral control of sensory afferents, mainly unmyelinated nociceptors and to a lesser extent neurons with Adelta or Abeta fibers, and in the control of cellular excitability of some dorsal root cell bodies through a paracrine mechanism of action.

Author information

Author/s: Van Steenwinckel, J (J); Noghero, A (A); Thibault, K (K); Brisorgueil, M-J (MJ); Fischer, J (J); Conrath, M (M);

Affiliation: Neurobiologie des Signaux Intercellulaires, CNRS UMR 7101, Université Pierre et Marie Curie, Paris, France.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Neuroscience (Neuroscience), published in United States. (Language: eng)

Reference: 2009-Jul; vol 161 (issue 3) : pp 838-46

Dates: Created 2009/06/01; Completed 2009/08/24;

PMID: 19362128, status: MEDLINE (last retrieval date: 8/24/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Cell Membrane - metabolism Cell Size Cytoplasm - metabolism Ganglia, Spinal - cytology; metabolism; ultrastructure Hydroxyindoleacetic Acid - metabolism Lectins - metabolism Lumbar Vertebrae Neurofilament Proteins - metabolism

Nociceptors - cytology; metabolism; ultrastructure Parvalbumins - metabolism Rats Rats, Wistar Receptor, Serotonin, 5-HT2A - metabolism Satellite Cells, Perineuronal - physiology; ultrastructure Serotonin - metabolism Substance P - metabolism TRPV Cation Channels - metabolism

Associated Chemicals: Lectins (0) ; Neurofilament Proteins (0) ; Parvalbumins (0) ; Receptor, Serotonin, 5-HT2A (0) ; TRPV Cation Channels (0) ; TRPV1 protein, mouse (0) ; neurofilament protein H (108688-71-7) ; Substance P (33507-63-0) ; Serotonin (50-67-9) ; Hydroxyindoleacetic Acid (54-16-0)

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