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| Research article summary (published 6 Jun 2009): |
Oral supplementation with melon superoxide dismutase extract promotes antioxidant defences in the brain and prevents stress-induced impairment of spatial memory.
Full Abstract
The purpose of this study was to investigate the effect of antioxidant ingestion on stress-induced impairment of cognitive memory. Male C57BL/6 mice were divided into four groups as follows: (1) control mice (C mice) fed in a normal cage without immobilization; (2) restraint-stressed (RS mice) fed in a small cage; (3) vitamin E mice (VE mice), mice were fed in a small cage with a diet supplemented with vitamin E; (4) GliSODin mice (GS mice) fed in a small cage with a diet supplemented with GliSODin. RS, VE and GS mice were exposed to 12 h of immobilization daily. Five weeks later, spatial learning was measured using the Morris Water Maze (MWM) test. After water maze testing, we performed immunohistochemical analysis using 4-hydroxy-2-noneral (4-HNE) and an anti-Ki67 antibody. 4-HNE is a marker of lipid peroxidation. RS mice showed impaired spatial learning performance and an increased number of 4-HNE-positive cells in the granule cell layer (GCL) of the hippocampal dentate gyrus when compared to C mice. Moreover, RS mice showed a decreased number of Ki67-positive cells in the subgranular zone (SGZ). GS mice showed better spatial learning memory than RS mice. The number of 4-HNE-positive cells in the GCL of GS mice was significantly less than that of RS mice. The number of Ki67-positive cells in the SGZ of GS mice was significantly greater than that of RS mice. These finding suggests that GliSODin prevents stress-induced impairment of cognitive function and maintains neurogenesis in the hippocampus through antioxidant activity.
Author information
Author/s: Nakajima, Sanae (S); Ohsawa, Ikuroh (I); Nagata, Kazufumi (K); Ohta, Shigeo (S); Ohno, Makoto (M); Ijichi, Tetsuo (T); Mikami, Toshio (T);
Affiliation: Department of Biochemistry and Cell Biology, Institute of Gerontology, Nippon Medical School, Kawasaki, Kanagawa 211-8533, Japan.
Journal and publication information
Publication Type: Journal Article
Journal: Behavioural brain research (Behav Brain Res), published in Netherlands. (Language: eng)
Reference: 2009-Jun; vol 200 (issue 1) : pp 15-21
Dates: Created 2009/04/16; Completed 2009/06/18;
PMID: 19373977, status: MEDLINE (last retrieval date: 6/18/2009, IMS Date: 18 Jun 2009 00:00:00)
Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.
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