How to predict implantation? No correlation between embryonic aneuploidy and soluble human leukocyte antigen G-concentrations.

Full Abstract

OBJECTIVE: To determine if soluble human leukocyte antigen-G (sHLA-G) concentrations in spent culture media may assist in identifying the normal embryo for implantation. DESIGN: Prospective blinded comparative study. SETTING: Reproductive genetic and reproductive medicine centers. PATIENT(S): One hundred and sixteen embryos obtained from eight patients undergoing in vitro fertilization (IVF) with preimplantation genetic diagnosis (PGD). INTERVENTION(S): Culture media obtained 2 days after fertilization were analyzed for sHLA-G concentrations using an enzyme-linked immunosorbent assay (ELISA) assay. A sHLA-G concentration of >or=1.9 mIU/mL was considered a positive predictor for successful implantation. Polar bodies and blastomeres from day-3 embryos were tested by PGD for 5 to 11 chromosomes: 8, 9, 13, 15, 16, 17, 18, 21, 22, X, and Y. MAIN OUTCOME MEASURE(S): The results of the sHLA-G concentrations were compared with the results of the PGD analyses. RESULT(S): We found an sHLA-G concentration >or=1.9 mIU/mL in 48% (56 out of 116) and normal PGD results in 52% (57 out of 116) of embryos. Of the embryos with normal PGD results, 46% (26 out of 57) had sHLA-G concentrations >or=1.9 mIU/mL. Among the embryos with sHLA-G >or=1.9 mIU/mL, 46% (26 out of 56) had normal PGD results, and 21% of embryos displayed both normal PGD results and sHLA-G >or=1.9 mIU/mL. CONCLUSION(S): No correlation between concentrations of sHLA-G in embryo culture media and PGD results of an embryo's aneuploidy were observed.

Author information

Author/s: Coulam, Carolyn B (CB); Roussev, Roumen G (RG); Lerner, Sveltana (S); Zlatopolsky, Zev (Z); Ilkevitch, Yuri (Y); Tur-Kaspa, Ilan (I);

Affiliation: Rinehart Center for Reproductive Medicine, Evanston, Illinois, USA. cbcoulam(-atsign-)aol.com

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Fertility and sterility (Fertil Steril), published in United States. (Language: eng)

Reference: 2009-Jun; vol 91 (issue 6) : pp 2408-13

Dates: Created 2009/06/01; Completed 2009/07/08;

PMID: 19394602, status: MEDLINE (last retrieval date: 7/24/2009, IMS Date: )

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