Research article summary (published 29 Apr 1991):

The effect of in-vitro ageing on mouse sperm chromosomes.

Full Abstract

In-vitro ageing has long been suspected to affect adversely the ability of spermatozoa to fertilize and produce viable zygotes. A comparison of the first cleavage chromosome complement of 824 mouse embryos fertilized with fresh spermatozoa and 656 embryos fertilized with spermatozoa aged for 12, 24 or 48 h is reported herein. The chromosome analysis of first-cleavage embryos allows us to study directly the paternal and maternal chromosome complements which contribute to the embryo. Both chromosome clusters remain separate when an antimitotic agent is used to prevent metaphase synchronization, allowing identification of maternal and paternal chromosomes. We show that after sperm ageing, there is a decrease in the fertilization rate from 75% in the controls to 57.5% (12 h of ageing), 63.5% (24h of ageing) and 4.4% (48 h of ageing). Simultaneously, the level of chromosome structural abnormalities increase from 1.3% in the controls to 16.5% after 12 h of ageing, 18.8% after 24 h of ageing and 59% after 48 h of ageing. The incidence of aneuploidy and polyploidy is not affected by ageing of the spermatozoa. Most of the structural abnormalities are paternal in origin and are presumably induced by the ageing of spermatozoa.

Author information

Author/s: Munne, S (S); Estop, A (A);

Affiliation: Department of Medical Genetics, West Penn Hospital, Pittsburgh 15224.

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't

Journal: Human reproduction (Oxford, England) (Hum Reprod), published in ENGLAND. (Language: eng)

Reference: 1991-May; vol 6 (issue 5) : pp 703-8

Dates: Created 1991/12/09; Completed 1991/12/09; Revised 2007/11/15;

PMID: 1939553, status: MEDLINE (last retrieved date: 2/18/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Animals Cell Aging Chromosome Aberrations Embryo, Mammalian - abnormalities Female Fertilization in Vitro

Fertilization in Vitro Karyotyping Male Mice Sperm-Ovum Interactions Spermatozoa - ultrastructure

Note: Bold headings indicate primary MeSH headings or qualifiers.

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