Research article summary (published May 2009):

Effect of chilling and cryopreservation on expression of Pax genes in zebrafish (Danio rerio) embryos and blastomeres.

Full Abstract

Cryopreservation is now common practice in the fields of aquaculture, conservation and biomedicine. However, there is a lack of information on the effect of chilling and cryopreservation at the molecular level. In the present study, we used real-time RT-PCR analysis to determine the effect of chilling and cryopreservation on expression of Pax2a, Pax2b, Pax5 and Pax8 which constitute one subgroup of the Pax gene family. As intact embryos of zebrafish have not yet been successfully cryopreserved, we have used two alternatives: chilling of intact embryos and cryopreservation of isolated blastomeres. Cryopreservation was found to affect the normal pattern of gene expression in zebrafish embryonic blastomeres. The trends, profile changes, in expression of Pax2a and Pax5 occurred to a lesser extent in frozen-thawed blastomeres than in fresh blastomeres whilst the opposite was true for Pax8. The trends in expression of Pax2b were delayed in frozen-thawed blastomeres compared to fresh blastomeres. Cryopreservation can therefore disrupt normal gene expression patterns in zebrafish embryonic blastomeres which could have a detrimental effect on embryo development.

Author information

Author/s: Lin, C (C); Spikings, E (E); Zhang, T (T); Rawson, D M (DM);

Affiliation: LIRANS Institute of Research in the Applied Natural Sciences, University of Bedfordshire, Bedfordshire, UK.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Cryobiology (Cryobiology), published in United States. (Language: eng)

Reference: 2009-Aug; vol 59 (issue 1) : pp 42-7

Dates: Created 2009/07/06; Completed 2009/09/10;

PMID: 19397904, status: MEDLINE (last retrieval date: 9/10/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Blastomeres - physiology Cold Temperature Cryopreservation - methods Cryoprotective Agents - pharmacology Embryo Culture Techniques Embryo, Nonmammalian - metabolism Female

Female Freezing Gene Expression Profiling Gene Expression Regulation, Developmental Male Paired Box Transcription Factors - metabolism Temperature Zebrafish

Associated Chemicals: Cryoprotective Agents (0) ; Paired Box Transcription Factors (0)

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