Phenotype and chondrogenic differentiation of mesenchymal cells from adipose tissue of different species.

Full Abstract

Mesenchymal stem cells (MSCs) are multipotent cells capable of differentiating into several mesoderm lineages. They have been isolated from different tissues, such as bone marrow, adult peripheral blood, umbilical cord blood, and adipose tissue. The aim of this study was to analyze the differences in proliferation and phenotype of adipose tissue-derived MSCs from three different species, and to evaluate their capacity to differentiate into chondrocytes in vitro. A comparative study of cultured human, rabbit, and sheep mesenchymal cells from adipose tissue was carried out, and the main morphological parameters, proliferative activity, and expression of surface markers were characterized. Proliferation and flow cytometry data showed species-related differences between animal and human MSCs. Histological staining suggested that rabbit and sheep mesenchymal cells were able to differentiate into chondrocytic lineages. Human mesenchymal cells, though they could also differentiate, accomplished it with more difficulty than animal MSCs. These results could help to explain the differences in the chondrogenic capacity of sheep and rabbit MSCs when they are used as animal models compared to human mesenchymal cells in a clinical assay. (c) 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.

Author information

Author/s: Martínez-Lorenzo, María José (MJ); Royo-Cañas, María (M); Alegre-Aguarón, Elena (E); Desportes, Paula (P); Castiella, Tomás (T); García-Alvarez, Felícito (F); Larrad, Luis (L);

Affiliation: Banco de Sangre y Tejidos de Aragón, Area de Tejidos, Zaragoza, Spain. mmartinezlor(-atsign-)salud.aragon.es

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society (J Orthop Res), published in United States. (Language: eng)

Reference: 2009-Nov; vol 27 (issue 11) : pp 1499-507

Dates: Created 2009/10/21; Completed 2009/11/06;

PMID: 19408284, status: MEDLINE (last retrieval date: 11/6/2009, IMS Date: )

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