Research article summary (published May 2009):

Dosage effects of EGb761 on hydrogen peroxide-induced cell death in SH-SY5Y cells.

Full Abstract

Standardized extract from the leaves of the Ginkgo biloba tree, labeled EGb761, is one of the most popular herbal supplements, taken for its multivalent properties. In this study, dosage effects of EGb761 on hydrogen peroxide (H(2)O(2))-induced apoptosis of human neuroblastoma SH-SY5Y cells were investigated. It was found that H(2)O(2)-induced apoptotic cell death in SH-SY5Y cells, which was revealed in DNA fragmentation, mitochondrial membrane potential depolarization, and activation of Akt, c-Jun N-terminal kinases (JNK) and caspase 3. Low doses of EGb761 (50-100 microg/ml) inhibited H(2)O(2)-induced cell apoptosis via inactivation of Akt, JNK and caspase 3 while high doses of EGb761 (250-500 microg/ml) enhanced H(2)O(2) toxicities via inactivation of Akt and enhancement of activation of JNK and caspase 3. Additional experiments revealed that H(2)O(2) decreased intracellular GSH content, which was also inhibited by low concentrations of EGb761 but enhanced after high concentrations of EGb761 treatment. This further suggests to us that dosage effects of EGb761 on apoptotic signaling proteins may be correlated with regulation of cell redox state. Therefore, treatment dosage may be one of the vital factors that determine the specific action of EGb761 on oxidative stress-induced cell apoptosis. To understand the mechanisms of dosage effects of EGb761 may have important clinical implications.

Author information

Author/s: Shi, Chun (C); Zhao, Lina (L); Zhu, Bilian (B); Li, Qianxi (Q); Yew, David T (DT); Yao, Zhibin (Z); Xu, Jie (J);

Affiliation: Department of Anatomy, Zhongshan School of Medicine, Sun Yat-Sen University Guangzhou, Guangdong 510080, China.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Chemico-biological interactions (Chem Biol Interact), published in Ireland. (Language: eng)

Reference: 2009-Aug; vol 180 (issue 3) : pp 389-97

Dates: Created 2009/06/22; Completed 2009/07/16;

PMID: 19414004, status: MEDLINE (last retrieval date: 7/25/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Apoptosis Caspase 3 - metabolism Cell Line, Tumor Dose-Response Relationship, Drug Ginkgo biloba - chemistry Glutathione - metabolism

Glutathione - metabolism Humans Hydrogen Peroxide - toxicity JNK Mitogen-Activated Protein Kinases - metabolism Plant Extracts - administration & dosage; pharmacology Proto-Oncogene Proteins c-akt - metabolism

Associated Chemicals: Ginkgo biloba extract 761 (0) ; Plant Extracts (0) ; Glutathione (70-18-8) ; Hydrogen Peroxide (7722-84-1) ; JNK Mitogen-Activated Protein Kinases (EC 2.7.1.37) ; Proto-Oncogene Proteins c-akt (EC 2.7.1.37) ; Caspase 3 (EC 3.4.22.-)

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