Research article summary (published 30 May 2009):

Emodin sensitizes paclitaxel-resistant human ovarian cancer cells to paclitaxel-induced apoptosis in vitro.

Full Abstract

Ovarian cancer has the highest mortality rate among gynecologic malignancies in the world, and the development of drug resistance is a major impediment toward successful treatment of the desease. Emodin has been reported to sensitize human tumor cells to chemotherapeutic agents. The present study investigated whether emodin could overcome chemoresistance of A2780/taxol cells. Cells were treated with different concentration of emodin alone or combined with paclitaxel, then the cell viability was measured by MTT and the apoptosis was determined by flow cytometric analysis. The changes of mRNA and protein were examined by QRT-PCR and Western blotting. The function of P-glycoprotein was also determined by flow cytometry. The results showed that emodin induced apoptosis alone at a high concentration and increased paclitaxel-induced apoptosis at a low concentration. It enhanced the sensitivity of A2780/taxol cells to paclitaxel with down-regulation of P-glycoprotein, XIAP and survivin. Taken together, the results demonstrated a dual role for emodin in the inhibition of drug resistant ovarian tumor growth by increasing paclitaxel cellular concentration and re-sensitizing the resistant cells to paclitaxel. Our results suggest the possibility of an innovative chemotherapeutic strategy that uses emodin in combination with paclitaxel to increase the sensitivity of tumor cells.

Author information

Author/s: Li, Juan (J); Liu, Peishu (P); Mao, Hongluan (H); Wanga, Ancong (A); Zhang, Xiaolei (X);

Affiliation: Department of Gynecology and Obstetrics, Qilu Hospital, Shandong University, Shandong 250012, P.R. China.

Journal and publication information

Publication Type: Journal Article

Journal: Oncology reports (Oncol Rep), published in Greece. (Language: eng)

Reference: 2009-Jun; vol 21 (issue 6) : pp 1605-10

Dates: Created 2009/05/08; Completed 2009/07/09;

PMID: 19424643, status: MEDLINE (last retrieval date: 7/24/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Antineoplastic Combined Chemotherapy Protocols - pharmacology Apoptosis - drug effects; genetics Cell Line, Tumor Cell Survival - drug effects Dose-Response Relationship, Drug Drug Resistance, Neoplasm Emodin - pharmacology Female

Gene Expression Regulation, Neoplastic - drug effects Humans Microtubule-Associated Proteins - metabolism Ovarian Neoplasms - genetics; metabolism; pathology P-Glycoprotein - metabolism Paclitaxel - pharmacology RNA, Messenger - metabolism X-Linked Inhibitor of Apoptosis Protein - metabolism

Associated Chemicals: ABCB1 protein, human (0) ; BIRC5 protein, human (0) ; Microtubule-Associated Proteins (0) ; P-Glycoprotein (0) ; RNA, Messenger (0) ; X-Linked Inhibitor of Apoptosis Protein (0) ; XIAP protein, human (0) ; Paclitaxel (33069-62-4) ; Emodin (518-82-1)

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