Expression of keratinocyte growth factor and its receptor in odontogenic keratocysts.

Full Abstract

BACKGROUND: The mitotic activity of the epithelial cells of odontogenic keratocysts (OKCs) is greater than that of other odontogenic jaw cysts, and the mitotic activity of the epithelial cells decreases after marsupialization. Keratinocyte growth factor (KGF) interacts with its specific receptor (KGFR), and elicits the proliferation and/or differentiation of the various types of epithelial cells. The aim of this study was to investigate the expression of KGF/KGFR in OKCs before and after marsupialization. METHODS: The expression of KGF was immunohistochemically detected in the specimens of 16 OKCs and 11 dentigerous cysts before and after marsupialization. The expression of KGF mRNA was measured in the fibroblasts isolated from OKCs by real-time PCR. RESULTS: KGF was expressed in the epithelial cells and fibroblasts of 12 and seven of 16 OKC specimens, respectively. The intensity of the KGF expression in both the epithelial cells and the fibroblasts significantly decreased after marsupialization. KGFR was expressed throughout the epithelium in 15 of 16 OKC specimens, but the intensity of the KGFR expression did not change after marsupialization. The expression of KGF was detected in the epithelium of two of 11 dentigerous cyst specimens, but not in the fibroblasts before marsupialization. Real-time PCR revealed that recombinant human interleukin (IL)-1alpha increased the expression of KGF mRNA in the fibroblasts isolated from OKCs. CONCLUSION: KGF/KGFR signaling may play a crucial role in the epithelial cells of OKCs. Furthermore, the expression of KGF in the fibroblasts of OKCs is regulated by IL-1alpha.

Author information

Author/s: Suyama, Y (Y); Kubota, Y (Y); Yamashiro, T (T); Ninomiya, T (T); Koji, T (T); Shirasuna, K (K);

Affiliation: Department of Oral and Maxillofacial Surgery, Graduate School of Dental Science, Kyushu University, Fukuoka, Japan.

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (J Oral Pathol Med), published in Denmark. (Language: eng)

Reference: 2009-May; vol 38 (issue 5) : pp 476-80

Dates: Created 2009/05/11; Completed 2009/09/24;

PMID: 19434818, status: MEDLINE (last retrieved date: 9/24/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Adolescent
Adult
Chi-Square Distribution
Dentigerous Cyst - metabolism; pathology
Epithelial Cells - metabolism
Female
Fibroblast Growth Factor 7 - metabolism
Humans

Humans
Immunohistochemistry
Interleukin-1alpha - metabolism
Male
Odontogenic Cysts - metabolism; pathology
Receptor, Fibroblast Growth Factor, Type 2 - metabolism
Statistics, Nonparametric
Young Adult

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: Interleukin-1alpha (0) ; Fibroblast Growth Factor 7 (126469-10-1) ; keratinocyte growth factor receptor (EC 2.7.1.-) ; Receptor, Fibroblast Growth Factor, Type 2 (EC 2.7.1.112)

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