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| Research article summary (published 30 May 2009): |
Systemic polyethylene glycol promotes neurological recovery and tissue sparing in rats after cervical spinal cord injury.
Full Abstract
Polyethylene glycol (PEG) has been reported to possess fusogenic properties that may confer neuroprotection after spinal cord injury (SCI), but there is uncertainty regarding the mechanisms of PEG in vivo and the robustness of its protective effects. We hypothesized that PEG promotes preservation of cytoskeletal proteins associated with white matter protection and neurobehavioral recovery after SCI. In proof-of-principle experiments using a pin-drop organotypic culture model of SCI, PEG attenuated neural cell death. Adult rats underwent 35-g clip compression SCI at C8 and were randomized postinjury to receive intravenous 30% PEG or sterile Ringer's lactate solution. Confocal microscopy and high-performance liquid chromatography of fluorescein-conjugated PEG permitted in vivo quantification of PEG concentrations in the injured and uninjured spinal cord. Western blot, immunohistochemistry, and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling staining demonstrated that PEG reduced 200-kd neurofilament degradation and apoptotic cell death. Polyethylene glycol also promoted spinal cord tissue sparing based on retrograde axonal Fluoro-Gold tracing and morphometric histological assessment. Polyethylene glycol also promoted significant, although modest, neurobehavioral recovery after SCI. Collectively, these results indicate that PEG protects key axonal cytoskeletal proteins after SCI, and that the protection is associated with axonal preservation. The modest extent of locomotor recovery after treatment with PEG suggests, however, that this compound may notconfer sufficient neuroprotection to be used clinically as a single treatment.
Author information
Author/s: Baptiste, Darryl C (DC); Austin, James W (JW); Zhao, William (W); Nahirny, Adrian (A); Sugita, Shuzo (S); Fehlings, Michael G (MG);
Affiliation: Division of Genetics & Development, Toronto Western Research Institute and Krembil Neuroscience Centre, Western Hospital, University Health Network, University of Toronto, Toronto, Ontario, Canada.
Journal and publication information
Publication Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't
Journal: Journal of neuropathology and experimental neurology (J Neuropathol Exp Neurol), published in United States. (Language: eng)
Reference: 2009-Jun; vol 68 (issue 6) : pp 661-76
Dates: Created 2009/05/29; Completed 2009/06/30;
PMID: 19458542, status: MEDLINE (last retrieval date: 6/30/2009, IMS Date: 30 Jun 2009 00:00:00)
Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.
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