GATA-3 is upregulated in peripheral blood mononuclear cells from patients with minimal change nephrotic syndrome.

Full Abstract

BACKGROUND: An imbalance of Th1 and Th2 cytokines has been reported in MCNS. Interleukin-13 (IL-13: Th2 cytokine) has been implicated in the pathogenesis of MCNS, but Th1/Th2 regulators such as T-bet (Th1-specific transcription factor) and GATA-3 (Th2-specific transcription factor) have not been examined. METHODS: We isolated PBMC from 25 patients with MCNS during nephrosis and remission phases, from 17 nephrotic patients with membranous nephropathy (MN), and from 25 healthy subjects. We measured mRNA expression levels of T-bet, GATA-3, Stat5A (regulator of Th2 priming), IFN-gamma (Th1 cytokine), IL-2 (Th1 cytokine and activator of Stat5), IL-4 (Th2 cytokine), and IL-13 in PBMC, using real-time RT-PCR. RESULTS: GATA-3, Stat5A, and IL-13 mRNA expression levels were higher in the nephrotic MCNS group compared to the others. IL-2 mRNA expression levels were higher in nephrotic patients with MCNS and MN than in MCNS patients in remission and healthy controls. There were no differences in mRNA expression levels of T-bet, IFN-gamma, and IL-4 between MCNS and MN patients and healthy controls. CONCLUSIONS: This study is the first to reveal increased mRNA expression levels of GATA-3 and Stat5A in PBMC from MCNS patients in nephrosis. This study also supports recent findings suggesting the role of IL-13 in the development of MCNS. A predominant Th2 type of T cell activation may be involved in the pathogenesis of MCNS.

Author information

Author/s: Komatsuda, A (A); Wakui, H (H); Iwamoto, K (K); Togashi, M (M); Masai, R (R); Maki, N (N); Sawada, K (K);

Affiliation: Third Department of Internal Medicine, Akita University School of Medicine, Akita, Japan. komatsud(-atsign-)med.akita-u-ac-jp

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't

Journal: Clinical nephrology (Clin Nephrol), published in Germany. (Language: eng)

Reference: 2009-Jun; vol 71 (issue 6) : pp 608-16

Dates: Created 2009/05/28; Completed 2009/07/23;

PMID: 19473628, status: MEDLINE (last retrieved date: 8/20/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Adolescent
Adult
Aged
Case-Control Studies
Female
GATA3 Transcription Factor - genetics; metabolism
Gene Expression
Glomerulonephritis, Membranous - genetics
Humans
Interferon-gamma - genetics
Interleukin-13 - genetics

Interleukin-2 - genetics
Interleukin-4 - genetics
Leukocytes, Mononuclear - metabolism
Male
Middle Aged
Nephrosis, Lipoid - genetics
RNA, Messenger - genetics
STAT5 Transcription Factor - genetics
T-Box Domain Proteins - genetics
Up-Regulation
Young Adult

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: GATA3 Transcription Factor (0) ; Interleukin-13 (0) ; Interleukin-2 (0) ; RNA, Messenger (0) ; STAT5 Transcription Factor (0) ; T-Box Domain Proteins (0) ; T-box transcription factor TBX21 (0) ; Interleukin-4 (207137-56-2) ; Interferon-gamma (82115-62-6)

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