An endophytic taxol-producing fungus from Taxus media, Cladosporium cladosporioides MD2.

Full Abstract

Fermentation processes using taxol-producing fungi other than Taxus spp. may be an alternative way to produce taxol, which is an important antitumor agent used widely in the clinic setting. In this study, a taxol-producing endophytic fungus strain MD2 was isolated from the inner bark of Taxus media. Strain MD2 produced taxol when grown in potato dextrose liquid medium. The fungal taxol-which was analyzed by ultraviolet, high-performance liquid chromatography and mass spectrometry-was shown to be identical to authentic taxol and 10-deacetylbaccatin III. Further analysis with nuclear magnetic resonance (NMR) spectroscopy to show the chemical structure of the fungal taxol indicated that the fungal taxol produced an NMR spectrum identical to that of authentic taxol. Strain MD2 was identified as Cladosporium cladosporioides according to morphology of the fungal culture, characteristics of the spores, and analysis of 18S rDNA sequence. In addition, 10-deacetylbaccatin III-10-O-acetyl transferase gene of C. cladosporioides MD2 was cloned for the first time and was shown to share 99% identity with that of T. x media and 97% identity with that of T. wallichiana var. mairei.

Author information

Author/s: Zhang, Peng (P); Zhou, Peng-Peng (PP); Yu, Long-Jiang (LJ);

Affiliation: Institute of Resource Biology and Biotechnology, Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Current microbiology (Curr Microbiol), published in United States. (Language: eng)

Reference: 2009-Sep; vol 59 (issue 3) : pp 227-32

Dates: Created 2009/08/11; Completed 2009/10/02;

PMID: 19484305, status: MEDLINE (last retrieval date: 10/2/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Acetyltransferases - genetics
Animals
Chromatography, High Pressure Liquid
Cladosporium - classification; genetics; isolation & purification; metabolism
Cloning, Molecular
Cluster Analysis
Culture Media - chemistry
DNA, Fungal - chemistry; genetics
DNA, Ribosomal - chemistry; genetics
Fungal Proteins - genetics
Genes, rRNA

Associated Chemicals: Culture Media (0) ; DNA, Fungal (0) ; DNA, Ribosomal (0) ; Fungal Proteins (0) ; RNA, Fungal (0) ; RNA, Ribosomal, 18S (0) ; Paclitaxel (33069-62-4) ; Acetyltransferases (EC 2.3.1.-)

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