Gene expression profile of primary prostate epithelial and stromal cells in response to sulforaphane or iberin exposure.

Full Abstract

BACKGROUND: Broccoli consumption has been associated with a reduced risk of prostate cancer. Isothiocyanates (ITCs) derived from glucosinolates that accumulate in broccoli are dietary compounds that may mediate these health effects. Sulforaphane (SF, 4-methylsulphinylbutyl ITC) derives from heading broccoli (calabrese) and iberin (IB, 3-methylsulphinypropyl ITC) from sprouting broccoli. While there are many studies regarding the biological activity of SF, mainly undertaken with cancerous cells, there are few studies associated with IB. METHODS: Primary epithelial and stromal cells were derived from benign prostatic hyperplasia tissue. Affymetrix U133 Plus 2.0 whole genome arrays were used to compare global gene expression between these cells, and to quantify changes in gene expression following exposure to physiologically appropriate concentrations of SF and IB. Ontology and pathway analyses were used to interpret results. Changes in expression of a subset of genes were confirmed by real-time RT-PCR. RESULTS: Global gene expression profiling identified epithelial and stromal-specific gene expression profiles. SF induced more changes in epithelial cells, whereas IB was more effective in stromal cells. Although IB and SF induced different changes in gene expression in both epithelial and stromal cells, these were associated with similar pathways, such as cell cycle and detoxification. Both ITCs increased expression of PLAGL1, a tumor suppressor gene, in stromal cells and suppressed expression of the putative tumor promoting genes IFITM1, CSPG2, and VIM in epithelial cells. CONCLUSION: These data suggest that IB and SF both alter genes associated with cancer prevention, and IB should be investigated further as a potential chemopreventative agent. Prostate 69: 1411-1421, 2009. (c) 2009 Wiley-Liss, Inc.

Author information

Author/s: Chambers, Karen F (KF); Bacon, James R (JR); Kemsley, E Katherine (EK); Mills, Robert D (RD); Ball, Richard Y (RY); Mithen, Richard F (RF); Traka, Maria H (MH);

Affiliation: Natural Products and Health Programme, Institute of Food Research, Norwich, UK.

Grants: (Agency:Biotechnology and Biological Sciences Research Council)

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: The Prostate (Prostate), published in United States. (Language: eng)

Reference: 2009-Sep; vol 69 (issue 13) : pp 1411-21

Dates: Created 2009/09/01; Completed 2009/09/23;

PMID: 19489030, status: MEDLINE (last retrieved date: 9/23/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Anticarcinogenic Agents - pharmacology
Cells, Cultured
Epithelial Cells - drug effects; pathology; physiology
Gene Expression - drug effects
Gene Expression Profiling
Humans
Isothiocyanates - pharmacology
Male

Male
Plant Extracts - pharmacology
Precancerous Conditions - drug therapy; genetics; pathology
Prostate - drug effects; pathology; physiology
Prostatic Hyperplasia - drug therapy; genetics; pathology
Prostatic Neoplasms - prevention & control
Stromal Cells - drug effects; pathology; physiology
Thiocyanates - pharmacology

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: Anticarcinogenic Agents (0) ; Isothiocyanates (0) ; Plant Extracts (0) ; Thiocyanates (0) ; sulforafan (4478-93-7) ; iberin (505-44-2)

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