Research article summary (published Jun 2009):

Metabolomics-oriented isolation and structure elucidation of 37 compounds including two anthocyanins from Arabidopsis thaliana.

Full Abstract

In order to conduct metabolomic studies in a model plant for genome research, such as Arabidopsis thaliana (Arabidopsis), it is a prerequisite to obtain structural information for the isolated metabolites from the plant of interest. In this study, we isolated metabolites of Arabidopsis in a relatively non-targeted way, aiming at the construction of metabolite standards and chemotaxonomic comparison. Anthocyanins (5 and 7) called A8 and A10 were isolated and their structures were elucidated as cyanidin 3-O-[2-O-(beta-D-xylopyranosyl)-6-O-(4-O-(beta-D-glucopyranosyl)-E-p-coumaroyl)-beta-D-glucopyranoside]-5-O-[6-O-(malonyl)-beta-D-glucopyranoside] and cyanidin 3-O-[2-O-(2-O-(E-sinapoyl)-beta-D-xylopyranosyl)-6-O-(4-O-(beta-D-glucopyranosyl)-E-p-coumaroyl)-beta-D-glucopyranoside]-5-O-[beta-D-glucopyranoside] from analyses of 1D NMR, 2D NMR ((1)H NMR, NOE, (13)C NMR, HMBC and HMQC), HRFABMS, FT-ESI-MS and GC-TOF-MS data. In addition, 35 known compounds, including six anthocyanins, eight flavonols, one nucleoside, one indole glucosinolate, four phenylpropanoids and a derivative, together with three indoles, one carotenoid, one apocarotenoid, three galactolipids, two chlorophyll derivatives, one steroid, one hydrocarbon, and two dicarboxylic acids, were also isolated and identified from their spectroscopic data.

Author information

Author/s: Nakabayashi, Ryo (R); Kusano, Miyako (M); Kobayashi, Makoto (M); Tohge, Takayuki (T); Yonekura-Sakakibara, Keiko (K); Kogure, Noriyuki (N); Yamazaki, Mami (M); Kitajima, Mariko (M); Saito, Kazuki (K); Takayama, Hiromitsu (H);

Affiliation: Graduate School of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Phytochemistry (Phytochemistry), published in United States. (Language: eng)

Reference: 2009-May; vol 70 (issue 8) : pp 1017-29

Dates: Created 2009/07/14; Completed 2009/08/07;

PMID: 19497599, status: MEDLINE (last retrieved date: 8/21/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Anthocyanins - chemistry; classification; isolation & purification Arabidopsis - chemistry Carotenoids - chemistry; isolation & purification Galactolipids - chemistry; isolation & purification

Galactolipids - chemistry; isolation & purification Glucosides - chemistry; isolation & purification Indoles - chemistry; pharmacology Molecular Structure

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: Anthocyanins (0) ; Galactolipids (0) ; Glucosides (0) ; Indoles (0) ; Carotenoids (36-88-4)

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