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| Research article summary (published 30 Aug 2009): |
Algorithms for the automated analysis of cellular dynamics within living fungal colonies.
Full Abstract
We present robust and efficient algorithms to automate the measurement of nuclear movement and germ tube extension rates in living fungal networks. The aim is to facilitate the understanding of the dynamics and regulation of nuclear migration in growing fungal colonies. The proposed methodology combines a cascade correlation filter to identify nuclear centers from which 2D nuclear velocities are determined and a level set algorithm for centerline extraction to monitor spore (conidial) germling growth. We show how the proposed cascaded filter improves spatial resolution in the presence of noise and is robust when fluorescently labeled nuclei with different intensities are in close proximity to each other. The performance of the filter is evaluated by simulation in comparison to the well known Rayleigh and Sparrow criteria, and experimental evidence is given from clusters of nuclei and nuclei undergoing mitotic division. The capabilities developed have enabled the robust and objective analysis of 10's of Gigabytes of image data that is being exploited by biological scientists.
Author information
Author/s: Angarita-Jaimes, N C (NC); Roca, M G M (MG); Towers, C E (CE); Read, N D (ND); Towers, D P (DP);
Affiliation: School of Mechanical Engineering, University of Leeds, Leeds LS29JT, United Kingdom. menncaj(-atsign-)leeds.ac.uk
Journal and publication information
Publication Type: Journal Article; Research Support, Non-U.S. Gov't
Journal: Cytometry. Part A : the journal of the International Society for Analytical Cytology (Cytometry A), published in United States. (Language: eng)
Reference: 2009-Sep; vol 75 (issue 9) : pp 768-80
Dates: Created 2009/08/19; Completed 2009/10/22;
PMID: 19504570, status: MEDLINE (last retrieval date: 10/22/2009, IMS Date: )
Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.
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