Research article summary (published 5 Jun 2009):

Behavioural and dopaminergic alterations induced by a low dose of WIN 55,212-2 in a conditioned place preference procedure.

Full Abstract

AIMS: This study investigated the role of the cannabinoid CB1 receptor agonist, WIN 55,212-2, on motor activity. Subsequently, the effects of a low, stimulatory dose of WIN 55,212-2 and cocaine, as a positive control, were evaluated using a conditioned place preference (CPP) procedure. Upon completion of CPP, in rats that had been treated with WIN 55,212-2, dopaminergic status and spontaneous and d-amphetamine-induced motor activity were assessed. MAIN METHODS: Sprague-Dawley rats were evaluated for habituated motor activity following WIN 55,212-2 (0, 0.1, 0.3, 1 mg/kg, i.p.) administration. A stimulatory dose of WIN 55,212-2 (0.1 mg/kg, i.p.) and cocaine (20 mg/kg, i.p.) was selected to assess CPP behaviour. Upon completion of CPP, in one group, tissue levels of dopamine and its metabolites were measured in distinct brain regions (dorsal striatum, nucleus accumbens, prefrontal cortex, amygdala, hippocampus) using High Performance Liquid Chromatography with electrochemical detection. In another group, spontaneous and D-amphetamine-induced motor activity was evaluated in an open-field apparatus. KEY FINDINGS: The lowest dose of WIN 55,212-2 increased motor activity but did not produce CPP. As expected, cocaine induced clear CPP. Dopaminergic status was increased in a region-specific way and motor activity was enhanced following a challenge of D-amphetamine in rats that had been administered with WIN 55,212-2 during conditioning. SIGNIFICANCE: A stimulatory effect of WIN 55,212-2 on motor activity was not accompanied by place preference. Upon completion of the CPP procedure, this dose was found to induce region-specific hyperdopaminergia along with a greater sensitivity to a subsequent challenge dose of D-amphetamine.

Author information

Author/s: Polissidis, Alexia (A); Chouliara, Olga (O); Galanopoulos, Andreas (A); Marselos, Marios (M); Papadopoulou-Daifoti, Zeta (Z); Antoniou, Katerina (K);

Affiliation: Department of Pharmacology, Medical School, University of Ioannina, 45110 Ioannina, Greece.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Life sciences (Life Sci), published in Netherlands. (Language: eng)

Reference: 2009-Jul; vol 85 (issue 5-6) : pp 248-54

Dates: Created 2009/07/14; Completed 2009/07/30;

PMID: 19508876, status: MEDLINE (last retrieval date: 8/21/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Avoidance Learning - drug effects Behavior, Animal - drug effects; physiology Benzoxazines - pharmacology Calcium Channel Blockers - pharmacology Cannabinoids - agonists Central Nervous System Stimulants - pharmacology Conditioning, Classical - drug effects Dextroamphetamine - pharmacology

Dextroamphetamine - pharmacology Dopamine - metabolism Dopamine Uptake Inhibitors - pharmacology Morpholines - pharmacology Motor Activity - drug effects Naphthalenes - pharmacology Orientation - drug effects Rats Rats, Sprague-Dawley

Associated Chemicals: Benzoxazines (0) ; Calcium Channel Blockers (0) ; Cannabinoids (0) ; Central Nervous System Stimulants (0) ; Dopamine Uptake Inhibitors (0) ; Morpholines (0) ; Naphthalenes (0) ; Win 55212-2 (134959-51-6) ; Dextroamphetamine (51-64-9)

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