Research article summary (published 7 Jun 2009):

Modulation of TLR2 protein expression by miR-105 in human oral keratinocytes.

Full Abstract

Mammalian biological processes such as inflammation, involve regulation of hundreds of genes controlling onset and termination. MicroRNAs (miRNAs) can translationally repress target mRNAs and regulate innate immune responses. Our model system comprised primary human keratinocytes, which exhibited robust differences in inflammatory cytokine production (interleukin-6 and tumor necrosis factor-alpha) following specific Toll-like receptor 2 and 4 (TLR-2/TLR-4) agonist challenge. We challenged these primary cells with Porphyromonas gingivalis (a Gram-negative bacterium that triggers TLR-2 and TLR-4) and performed miRNA expression profiling. We identified miRNA (miR)-105 as a modulator of TLR-2 protein translation in human gingival keratinocytes. There was a strong inverse correlation between cells that had high cytokine responses following TLR-2 agonist challenge and miR-105 levels. Knock-in and knock-down of miR-105 confirmed this inverse relationship. In silico analysis predicted that miR-105 had complementarity for TLR-2 mRNA, and the luciferase reporter assay verified this. Further understanding of the role of miRNA in host responses may elucidate disease susceptibility and suggest new anti-inflammatory therapeutics.

Author information

Author/s: Benakanakere, Manjunatha R (MR); Li, Qiyan (Q); Eskan, Mehmet A (MA); Singh, Amar V (AV); Zhao, Jiawei (J); Galicia, Johnah C (JC); Stathopoulou, Panagiota (P); Knudsen, Thomas B (TB); Kinane, Denis F (DF);

Affiliation: Center for Oral Health and Systemic Disease, University of Louisville School of Dentistry, Louisville, Kentucky 40202, USA.

Grants: DE017384 (Agency:NIDCR NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, N.I.H., Extramural

Journal: The Journal of biological chemistry (J Biol Chem), published in United States. (Language: eng)

Reference: 2009-Aug; vol 284 (issue 34) : pp 23107-15

Dates: Created 2009/08/17; Completed 2009/10/06; Revised 2009/10/14;

PMID: 19509287, status: MEDLINE (last retrieved date: 10/15/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article
(including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Blotting, Western Cells, Cultured Gene Expression Profiling Gene Expression Regulation Humans Immunohistochemistry Interleukin-12 Subunit p40 - genetics

Interleukin-6 - genetics Keratinocytes - drug effects; metabolism MicroRNAs - antagonists & inhibitors; genetics; physiology Oligonucleotides - pharmacology Polymerase Chain Reaction Toll-Like Receptor 2 - genetics; metabolism Tumor Necrosis Factor-alpha - genetics

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: Interleukin-12 Subunit p40 (0) ; Interleukin-6 (0) ; MicroRNAs (0) ; Oligonucleotides (0) ; Toll-Like Receptor 2 (0) ; Tumor Necrosis Factor-alpha (0)

Related articles

These are the most related articles currently in our database:

• CBT-SL5, a bacteriocin from Enterococcus faecalis, suppresses the expression of interleukin-8 induced by Propionibacterium acnes in cultured human keratinocytes. 29 Jun 2008
• Low molecular weight hyaluronic acid increases the self-defense of skin epithelium by induction of beta-defensin 2 via TLR2 and TLR4. 30 Jul 2008
• Zinc salts inhibit in vitro Toll-like receptor 2 surface expression by keratinocytes. 17 Oct 2007
• Pimecrolimus enhances TLR2/6-induced expression of antimicrobial peptides in keratinocytes. 20 May 2008
• Expression, subcellular localization and cytokinic modulation of Toll-like receptors (TLRs) in normal human keratinocytes: TLR2 up-regulation in psoriatic skin. 17 Oct 2007
• Induction of beta-defensin 3 in keratinocytes stimulated by bacterial lipopeptides through toll-like receptor 2. 5 Apr 2006
• A skin microRNA promotes differentiation by repressing 'stemness'. 29 Feb 2008
• The imprinted H19 noncoding RNA is a primary microRNA precursor. 17 Jan 2007
• Innate immune modulation of keratinocytes by antikeratin 16 antibodies. 26 Jun 2008
• [Effect of topical tacrolimus ointment on expression of Toll-like receptors 2 and 4 in lesional atopic dermatitis skin] 16 Aug 2006

See 100+ related articles.

Related Article Map

Legend: - FREE Full text Article. - Abstract only. - Title only. More help.

See a larger map of 100+ related articles.