Research article summary (published 22 Mar 2009):

Emodin suppresses cell proliferation and fibronectin expression via p38MAPK pathway in rat mesangial cells cultured under high glucose.

Full Abstract

Our previous findings demonstrated that emodin could improve the renal function in rats with diabetic nephropathy, but little is known about its molecular mechanisms. In this study, we investigated the effects of emodin on high glucose (HG)-induced cell proliferation and fibronectin (FN) protein expression in rat mesangial cells, and explored the possible mechanism. Cell proliferation and cell cycle were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry assay, respectively. The protein levels of FN, p-p38MAPK, t-p38MAPK, p-CREB, PPARgamma, and CTGF in rat mesangial cells were detected by Western blot. Our results demonstrated that emodin significantly suppressed HG-induced cell proliferation and arrested cell cycle progress. Protein expression of FN, phospho-p38MAPK, phospho-CREB and CTGF was markedly reduced, and PPARgamma protein level was significantly increased after emodin treatment. In conclusion, emodin suppressed HG-induced cell proliferation and FN expression in rat mesangial cells through inhibiting the p38MAPK pathway involved CREB, PPAPgamma and CTGF, suggesting a potential role of emodin in the treatment of diabetic nephropathy.

Author information

Author/s: Li, Xuejuan (X); Liu, Weihua (W); Wang, Qin (Q); Liu, Peiqing (P); Deng, Yanhui (Y); Lan, Tian (T); Zhang, Xiaoyan (X); Qiu, Baoming (B); Ning, Hairong (H); Huang, Heqing (H);

Affiliation: School of Pharmaceutical Sciences, Sun Yat-Sen University, Guagnzhou, Guangdong, China.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Molecular and cellular endocrinology (Mol Cell Endocrinol), published in Ireland. (Language: eng)

Reference: 2009-Aug; vol 307 (issue 1-2) : pp 157-62

Dates: Created 2009/06/15; Completed 2009/08/28;

PMID: 19524136, status: MEDLINE (last retrieval date: 8/28/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Cell Cycle - drug effects Cell Proliferation - drug effects Cells, Cultured Connective Tissue Growth Factor - metabolism Cyclic AMP Response Element-Binding Protein - metabolism Emodin - pharmacology

Fibronectins - metabolism Fibrosis Glucose - pharmacology Mesangial Cells - cytology; drug effects; enzymology; pathology PPAR gamma - metabolism Rats p38 Mitogen-Activated Protein Kinases - metabolism

Associated Chemicals: Cyclic AMP Response Element-Binding Protein (0) ; Fibronectins (0) ; PPAR gamma (0) ; Connective Tissue Growth Factor (139568-91-5) ; Glucose (50-99-7) ; Emodin (518-82-1) ; p38 Mitogen-Activated Protein Kinases (EC 2.7.1.37)

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