Research article summary (published 13 Sep 2009):

MicroRNA-10b is overexpressed in malignant glioma and associated with tumor invasive factors, uPAR and RhoC.

Full Abstract

MicroRNAs (miRNAs) are effective post-transcriptional regulators of gene expression and are important in many biological processes. Although the oncogenic and tumor suppressive functions of several miRNAs have been characterized, the role of miRNAs in mediating tumor invasion and migration remains largely unexplored. Recently, miR-10b was identified as an miRNA highly expressed in metastatic breast cancer, promoting cell migration and invasion. Here, we performed real-time reverse transcriptase polymerase chain reaction (RT-PCR) assays on 43 glioma samples (17 glioblastoma, 6 anaplastic astrocytoma, 10 low-grade astrocytoma, 6 oligodendroglioma and 4 ependymoma) and 6 glioma cell lines. We found that miR-10b expression was upregulated in all glioma samples compared to non-neoplastic brain tissues. The expression levels of miR-10b were associated with higher grade glioma. In addition, mRNA expressions of RhoC and urokinase-type plasminogen activator receptor (uPAR), which were thought to be regulated by miR-10b via HOXD10, were statistically significantly correlated with the expression of miR-10b (p < 0.001, p = 0.001, respectively). Also, protein expression levels of RhoC and uPAR were associated with expression levels of miR-10b (p = 0.009, p = 0.014, respectively). Finally, multifocal lesions on enhanced MRI of 7 malignant gliomas were associated with higher expression levels of miR-10b (p = 0.02). Our data indicated that miR-10b might play some role in the invasion of glioma cells. 2009 UICC

Author information

Author/s: Sasayama, Takashi (T); Nishihara, Masamitsu (M); Kondoh, Takeshi (T); Hosoda, Kohkichi (K); Kohmura, Eiji (E);

Affiliation: Department of Neurosurgery, Kobe University Graduate School of Medicine, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe, Japan. takasasa(-atsign-)med.kobe-u.ac.jp

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: International journal of cancer. Journal international du cancer (Int J Cancer), published in United States. (Language: eng)

Reference: 2009-Sep; vol 125 (issue 6) : pp 1407-13

Dates: Created 2009/07/21; Completed 2009/08/20;

PMID: 19536818, status: MEDLINE (last retrieval date: 8/21/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Brain Neoplasms - genetics; pathology Gene Expression Regulation, Neoplastic - physiology Glioma - genetics; pathology Humans Immunoenzyme Techniques Membrane Glycoproteins - genetics Neoplasm Invasiveness Neoplasm Staging Oligonucleotide Array Sequence Analysis

Oligonucleotide Array Sequence Analysis RNA, Messenger - genetics; metabolism Receptors, Immunologic - genetics Receptors, Urokinase Plasminogen Activator - genetics Reverse Transcriptase Polymerase Chain Reaction Tumor Cells, Cultured Tumor Markers, Biological - genetics; metabolism Up-Regulation rho GTP-Binding Proteins - genetics

Associated Chemicals: LILRB2 protein, human (0) ; Membrane Glycoproteins (0) ; RHOC protein, human (0) ; RNA, Messenger (0) ; Receptors, Immunologic (0) ; Receptors, Urokinase Plasminogen Activator (0) ; Tumor Markers, Biological (0) ; rho GTP-Binding Proteins (EC 3.6.5.2)

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