Research article summary (published 13 Oct 2009):

The isolation and identification of a secreted biomarker associated with cell stress in serum-free CHO cell culture.

Full Abstract

One approach to improving mammalian culture productivity has been to reduce cell stress and cell death in the bioreactor, thus enhancing productivity through a longer phase of viability. Here we describe the isolation and identification of a biomarker for stress and viability loss in CHO culture. Using SELDI-TOF mass spectrometry to profile the protein component of supernatant culture media we have identified a peak at 7.7 kDa that was associated with loss of viability toward the end of the culture and simulated stress from both toxic metabolite accumulation and nutrient depletion. The relative intensity (signal/noise ratio) of the peak increased rapidly at the onset of dropping viability toward the end of the growth phase. Also, the peak height was seen to increase significantly when cells were grown under conditions emulating ammonia accumulation and glutamine deprivation. The species has been identified as a fragment of Galectin-1 (Gal-1) via MS/MS fingerprinting. We propose that this peak could be utilized as a marker for early onset of stress in cell culture. This work demonstrates the efficacy of SELDI technology to identify biomarkers in mammalian cell culture and highlights its value as a tool for the monitoring and improvement of culture processes.

Author information

Author/s: Woolley, John F (JF); Al-Rubeai, Mohamed (M);

Affiliation: School of Chemical and Bioprocess Engineering, and Conway Institute for Biomolecular and Biomedical Research, University College Dublin, Belfield, Dublin 4, Ireland.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Biotechnology and bioengineering (Biotechnol Bioeng), published in United States. (Language: eng)

Reference: 2009-Oct; vol 104 (issue 3) : pp 590-600

Dates: Created 2009/08/31; Completed 2009/10/22;

PMID: 19544303, status: MEDLINE (last retrieval date: 10/22/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article
(including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Biological Markers CHO Cells Cell Survival Cricetinae Cricetulus

Cricetulus Culture Media, Serum-Free - chemistry Galectin 1 - chemistry; secretion Mass Spectrometry Molecular Weight Stress, Physiological

Associated Chemicals: Biological Markers (0) ; Culture Media, Serum-Free (0) ; Galectin 1 (0)

Related articles

These are the highest related articles currently in the database:

• Membrane chromatography for rapid purification of recombinant antithrombin III and monoclonal antibodies from cell culture supernatant. 2 Jun 1993
• Specific amino acid profile in culture media conditioned by human pancreatic cancer cell lines. 30 Dec 2001
• Yeast hydrolysate as a low-cost additive to serum-free medium for the production of human thrombopoietin in suspension cultures of Chinese hamster ovary cells. 9 Jul 2003
• Application of a statistical design to the optimization of culture medium for recombinant interferon-gamma production by Chinese hamster ovary cells. 29 Sep 1992
• Development of a serum-free medium for the production of erythropoietin by suspension culture of recombinant Chinese hamster ovary cells using a statistical design. 13 Apr 1999
• Cationic liposome and plasmid DNA complexes formed in serum-free medium under optimum transfection condition are negatively charged. 30 May 2000
• Measurement of the tensile strength of cell-biomaterial interface using the laser spallation technique. 22 Jun 2008
• Apolipoprotein E and beta-amyloid (1-42) regulation of glycogen synthase kinase-3beta. 29 Nov 2003
• Osteogenic behavior of alginate encapsulated bone marrow stromal cells: an in vitro study. 28 Nov 2006
• Characterization of different cell culture media for expression of recombinant antibodies in mammalian cells: Presence of contaminating bovine antibodies. 30 May 2005

See 100+ related articles.

Related Article Map

Legend: - FREE Full text Article. - Abstract only. - Title only. More help.

See a large map of 100+ related articles.