Research article summary (published 13 Oct 2009):

Enrichment of anaerobic methanotrophs in sulfate-reducing membrane bioreactors.

Full Abstract

Anaerobic oxidation of methane (AOM) in marine sediments is coupled to sulfate reduction (SR). AOM is mediated by distinct groups of archaea, called anaerobic methanotrophs (ANME). ANME co-exist with sulfate-reducing bacteria, which are also involved in AOM coupled SR. The microorganisms involved in AOM coupled to SR are extremely difficult to grow in vitro. Here, a novel well-mixed submerged-membrane bioreactor system is used to grow and enrich the microorganisms mediating AOM coupled to SR. Four reactors were inoculated with sediment sampled in the Eckernförde Bay (Baltic Sea) and operated at a methane and sulfate loading rate of 4.8 L L(-1) day(-1) (196 mmol L(-1) day(-1)) and 3.0 mmol L(-1) day(-1). Two bioreactors were controlled at 15 degrees C and two at 30 degrees C, one reactor at 30 degrees C contained also anaerobic granular sludge. At 15 degrees C, the volumetric AOM and SR rates doubled approximately every 3.8 months. After 884 days, an enrichment culture was obtained with an AOM and SR rate of 1.0 mmol g(volatile suspended solids) (-1) day(-1) (286 micromol g(dry weight) (-1) day(-1)). No increase in AOM and SR was observed in the two bioreactors operated at 30 degrees C. The microbial community of one of the 15 degrees C reactors was analyzed. ANME-2a became the dominant archaea. This study showed that sulfate reduction with methane as electron donor is possible in well-mixed bioreactors and that the submerged-membrane bioreactor system is an excellent system to enrich slow-growing microorganisms, like methanotrophic archaea.

Author information

Author/s: Meulepas, Roel J W (RJ); Jagersma, Christian G (CG); Gieteling, Jarno (J); Buisman, Cees J N (CJ); Stams, Alfons J M (AJ); Lens, Piet N L (PN);

Affiliation: Sub-department of Environmental Technology, Wageningen University, Bomenweg 2, 6703 HD Wageningen, The Netherlands. r.meulepas(-atsign-)unesco-ihe.org

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Biotechnology and bioengineering (Biotechnol Bioeng), published in United States. (Language: eng)

Reference: 2009-Oct; vol 104 (issue 3) : pp 458-70

Dates: Created 2009/08/31; Completed 2009/10/22;

PMID: 19544305, status: MEDLINE (last retrieval date: 10/22/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Anaerobiosis Archaea - classification; growth & development; metabolism Bacteria - classification; growth & development; metabolism Biodiversity Bioreactors - microbiology DNA, Archaeal - chemistry; genetics DNA, Bacterial - chemistry; genetics DNA, Ribosomal - chemistry; genetics Genes, rRNA Geologic Sediments - microbiology Membranes - microbiology

Methane - metabolism Molecular Sequence Data Oxidation-Reduction Phylogeny RNA, Archaeal - genetics RNA, Bacterial - genetics RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Sequence Homology, Nucleic Acid Sulfates - metabolism Temperature

Associated Chemicals: DNA, Archaeal (0) ; DNA, Bacterial (0) ; DNA, Ribosomal (0) ; RNA, Archaeal (0) ; RNA, Bacterial (0) ; RNA, Ribosomal, 16S (0) ; Sulfates (0) ; Methane (74-82-8)

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