Research article summary (published 19 Jun 2009):

A novel nanoparticulate adjuvant for immunotherapy with Lolium perenne.

Full Abstract

Specific immunotherapy implies certain drawbacks which could be minimized by the use of appropriate adjuvants, capable of amplifying the right immune response with minimal side effects. In this context, previous studies of our group have demonstrated the adjuvant capacity of Gantrez AN nanoparticles, which can effectively enhance the immune response. In this work, two types of nanoparticles (with and without LPS of Brucella ovis as immunomodulator) with encapsulated Lolium perenne extract are tested in a model of sensitized mice to this allergenic mixture. The results we obtained showed that Lolium-Gantrez nanoparticles with LPS of B. ovis were able to induce significative Th1 responses, characterized by the IgG(2a) isotype. Furthermore, in the challenge experiment of the sensitized mice, differences in the mortality rate and in the mMCP-1 levels were found between the treated groups and the control. Under the experimental conditions of this model of pre-sensitized mice to L. perenne, Gantrez AN nanoparticles appeared to be a good strategy for immunotherapy.

Author information

Author/s: Gómez, Sara (S); Gamazo, Carlos (C); San Roman, Beatriz (B); Grau, Alicia (A); Espuelas, Socorro (S); Ferrer, Marta (M); Sanz, Maria L (ML); Irache, Juan M (JM);

Affiliation: Adjuvant Unit, Department of Pharmaceutical Technology and Microbiology, University of Navarra, 31080 Pamplona, Spain.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of immunological methods (J Immunol Methods), published in Netherlands. (Language: eng)

Reference: 2009-Aug; vol 348 (issue 1-2) : pp 1-8

Dates: Created 2009/08/18; Completed 2009/09/21;

PMID: 19545572, status: MEDLINE (last retrieval date: 9/21/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adjuvants, Immunologic - administration & dosage Allergens - administration & dosage Anaphylaxis - immunology; therapy Animals Antibodies, Bacterial - blood Basophils - immunology; metabolism Brucella ovis - immunology Chemokine CCL2 - blood Desensitization, Immunologic Drug Carriers Female Histamine - blood

Histamine - blood Immunoglobulin E - blood Lipopolysaccharides - immunology Lolium - immunology Maleates - chemistry Mice Mice, Inbred BALB C Nanoparticles - administration & dosage; chemistry; ultrastructure Pollen - immunology Polyvinyls - chemistry Th1 Cells - immunology; metabolism Th2 Cells - immunology; metabolism

Associated Chemicals: Adjuvants, Immunologic (0) ; Allergens (0) ; Antibodies, Bacterial (0) ; Ccl2 protein, mouse (0) ; Chemokine CCL2 (0) ; Drug Carriers (0) ; Lipopolysaccharides (0) ; Maleates (0) ; Polyvinyls (0) ; polyvinylmethoxyethylene-maleic anhydride copolymer (0) ; Immunoglobulin E (37341-29-0) ; Histamine (51-45-6)

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