Relative contributions of collagen and tissue factor to thrombus formation on damaged vascular vessels: in-vitro studies with circulating blood.

Full Abstract

BACKGROUND AND OBJECTIVE: Thrombogenicity of ruptured atherosclerotic plaques is mainly attributed to the exposure of collagen (Col) and tissue factor (TF). Using in-vitro approaches, we explored the relative contribution of Col and TF to local thrombogenesis. METHODS: Surfaces coated with Col and human TF, alone or in combinations (diluted Col on TF, and diluted TF on Col), were exposed to flowing blood at a shear rate of 600/s. Platelet and fibrin deposition were analyzed morphometrically. Generation of prothrombin fragments (F1+2) was determined as a measure of global activation of coagulation. RESULTS: Col and TF alone behaved as adhesive substrata to platelets supporting similar platelet coverage around 22%. Col induced tight aggregates, whereas TF promoted adhesive events. In studies with combinations of Col and TF, platelet aggregation was always enhanced with statistical elevations of the thrombus area (P<0.05). Generation of F1+2 was surface-dependent and was at its highest levels when both proteins were combined (P<0.05). However, local fibrin formation was statistically increased in surfaces containing lower concentrations of TF on Col (P<0.01 vs. overall surfaces assessed). CONCLUSION: Our studies show that combinations of Col and TF always enhance thrombogenesis, but small amounts of TF on Col surfaces resulted in the most procoagulant combination. The present results suggest that plaques exposing high contents of Col, with small amounts of TF, would provide the most occlusive combination.

Author information

Author/s: Lopez-Vilchez, Irene (I); Tonda, Raul (R); Hernandez, Rosa M (RM); Navalon, Fulgencio (F); Diaz-Ricart, Maribel (M); Galan, Ana M (AM); Escolar, Gines (G);

Affiliation: Servei d'Hemoterapia i Hemostasia, Hospital Clinic, Centre de Diagnostic Biomedic, Institut d'Investigacions Biomediques August Pi i Sunyer, Universitat de Barcelona, Barcelona, Spain. ilopez1(-atsign-)clinic.ub.es

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Coronary artery disease (Coron Artery Dis), published in England. (Language: eng)

Reference: 2009-Sep; vol 20 (issue 6) : pp 392-9

Dates: Created 2009/08/21; Completed 2009/10/29;

PMID: 19561489, status: MEDLINE (last retrieved date: 10/29/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Blood Coagulation
Blood Platelets - metabolism; ultrastructure
Blood Vessels - metabolism; physiopathology
Fibrillar Collagens - metabolism; ultrastructure
Fibrin - metabolism
Humans
Peptide Fragments - blood
Perfusion

Platelet Adhesiveness
Platelet Aggregation
Prothrombin
Pulsatile Flow
Rupture
Stress, Mechanical
Thromboplastin - metabolism
Thrombosis - blood; pathology; physiopathology

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: Fibrillar Collagens (0) ; Peptide Fragments (0) ; prothrombin fragment 1.2 (0) ; Prothrombin (9001-26-7) ; Fibrin (9001-31-4) ; Thromboplastin (9035-58-9)

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