Research article summary (published 29 Sep 2009):

Thyroid hormones induce cell proliferation and survival in ovarian granulosa cells COV434.

Full Abstract

Numerous evidences indicate that thyroid hormones exert an important role in the regulation of the reproductive system in the adult female. Although a clear demonstration of the thyroid-ovarian interaction is still lacking, it is conceivable that thyroid hormones might have a direct role in ovarian physiology via receptors in granulosa cells. In this study we analyzed if thyroid hormone treatment could affect cell proliferation and survival of COV434 cells. To this aim cell growth experiments and cell cycle analyses by flow cytometry were performed. Secondly the T(3) survival action was tested by TUNEL assay and MD30 cleavage analysis. We showed that T(3), and not T(4), can protect ovarian granulosa cells COV434 from apoptosis, regulating cell cycle and growth in the same cells. The increase in cell growth resulted in an augmented percentage of the cells in the S phase and, in a reduction of the doubling time (18%). Subsequently apoptotic pathway induced by serum deprivation has been evaluated in the cells exposed or not to thyroid hormone treatment. The T(3) treatment was able to remarkably counteract the apoptotic process. Even at the ultrastructural level there was an evident protective effect of T(3) in the cells that, besides the maintenance of the original morphology and, the absence of basophilic cytoplasm, conserved normal junctional areas. Furthermore, the protective T(3) effect evaluated by FACS analysis in the presence of a PI3K inhibitor revealed, as also confirmed by Western Blot on pAkt, that the PI3K pathway is crucial in T(3) survival action. Copyright 2009 Wiley-Liss, Inc.

Author information

Author/s: Verga Falzacappa, Cecilia (C); Mangialardo, Claudia (C); Patriarca, Valentina (V); Bucci, Barbara (B); Amendola, Donatella (D); Raffa, Salvatore (S); Torrisi, Maria Rosaria (MR); Silvestrini, Giuliana (G); Ballanti, Paola (P); Moriggi, Giulia (G); Stigliano, Antonio (A); Brunetti, Ercole (E); Toscano, Vincenzo (V); Misiti, Silvia (S);

Affiliation: II Faculty of Medicine, Sapienza, University of Rome, Rome, Italy.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of cellular physiology (J Cell Physiol), published in United States. (Language: eng)

Reference: 2009-Oct; vol 221 (issue 1) : pp 242-53

Dates: Created 2009/07/29; Completed 2009/08/28;

PMID: 19562675, status: MEDLINE (last retrieval date: 8/28/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

1-Phosphatidylinositol 3-Kinase - metabolism Apoptosis - drug effects Caspase 9 - metabolism Cell Cycle - drug effects Cell Line, Tumor Cell Proliferation - drug effects Cell Shape - drug effects Cell Survival - drug effects

Cytoprotection - drug effects Female Granulosa Cells - cytology; drug effects; enzymology; ultrastructure Humans Keratin-18 - metabolism Proto-Oncogene Proteins c-akt - metabolism Thyroxine - pharmacology Triiodothyronine - pharmacology

Associated Chemicals: Keratin-18 (0) ; Triiodothyronine (6893-02-3) ; Thyroxine (7488-70-2) ; 1-Phosphatidylinositol 3-Kinase (EC 2.7.1.137) ; Proto-Oncogene Proteins c-akt (EC 2.7.1.37) ; Caspase 9 (EC 3.4.22.-)

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