Research article summary (published 29 Jun 2009):

Profiling of polar metabolites in biological extracts using diamond hydride-based aqueous normal phase chromatography.

Full Abstract

Highly polar metabolites, such as sugars and most amino acids are not retained by conventional RP LC columns. Without sufficient retention low concentration compounds are not detected due ion suppression and structural isomers are not resolved. In contrast, hydrophilic interaction chromatography (HILIC) and aqueous normal phase chromatography (ANP) retain compounds based on their hydrophilicity and therefore provides a means of separating highly polar compounds. Here, an ANP method based on the diamond hydride stationary phase is presented for profiling biological small molecules by LC. A rapid separation system based upon a fast gradient that delivers reproducible chromatography is presented. Approximately 1000 compounds were reproducibly detected in human urine samples and clear differences between these samples were identified. This chromatography was also applied to xylem fluid from soyabean (Glycine max) plants to which 400 compounds were detected. This method greatly increases the metabolite coverage over RP-only metabolite profiling in biological samples. We show that both forms of chromatography are necessary for untargeted comprehensive metabolite profiling and that the diamond hydride stationary phase provides a good option for polar metabolite analysis.

Author information

Author/s: Callahan, Damien L (DL); De Souza, David (D); Bacic, Antony (A); Roessner, Ute (U);

Affiliation: Metabolomics Australia, The University of Melbourne, Victoria, Australia. damienlc(-atsign-)unimelb.edu.au

Journal and publication information

Publication Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of separation science (J Sep Sci), published in Germany. (Language: eng)

Reference: 2009-Jul; vol 32 (issue 13) : pp 2273-80

Dates: Created 2009/07/06; Completed 2009/09/14;

PMID: 19569107, status: MEDLINE (last retrieval date: 9/14/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Chromatography, Liquid - instrumentation; methods Coffee - chemistry; metabolism Diamond - chemistry Humans Hydrogen - chemistry Mass Spectrometry - instrumentation; methods

Molecular Structure Plant Extracts - chemistry Reproducibility of Results Soybeans - chemistry Urine - chemistry Vitamins - chemistry; metabolism

Associated Chemicals: Coffee (0) ; Plant Extracts (0) ; Vitamins (0) ; Hydrogen (1333-74-0) ; Diamond (7782-40-3)

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