Research article summary (published 13 Nov 2009):

Precise detection of lymph node metastases in mouse rectal cancer by using 5-aminolevulinic acid.

Full Abstract

Accurate diagnosis of metastatic lymph nodes (LNs) is essential in choosing appropriate treatment for gastrointestinal carcinoma. Our aim was to evaluate the diagnostic power of 5-aminolevulinic acid (5-ALA) for LN metastasis in mouse rectal cancer. Colorectal cancer cell lines, isolated cells from normal LNs, and orthotopic mouse model incorporating enhanced green fluorescent protein-tagged and untagged human rectal cancer cells were studied after 5-ALA administration by using confocal microscopy, fluorescence stereomicroscopy, fluorescence lifetime imaging microscopy (FLIM), multichannel spectrophotometry and macroconfocal imaging system to precisely detect LN metastases. In vitro confocal microscopic analyses showed that all colorectal cancer cell lines tested were positive for 5-ALA-induced fluorescence, whereas isolated normal LN cells were negative. 5-ALA-induced protoporphyrin IX (PPIX) fluorescence, verified by FLIM and multichannel spectrophotometry, revealed LN metastases in mice-bearing human rectal cancer cells. Occult LN metastases, unrecognized on white-light imaging and simplified hematoxylin-eosin analyses, were readily detectable on 5-ALA-induced PPIX fluorescence imaging. In vivo macroconfocal images clearly revealed PPIX-fluorescence-positive cancer cells in draining lymph vessels and nodes. Together with specific speckled patterns of PPIX-fluorescence in metastatic lesions, the PPIX-fluorescence intensity ratio of metastatic and nonmetastatic lesions discriminated metastasis with 100% sensitivity and 100% specificity in excised whole LN samples. These results show that fluorescence diagnosis with 5-ALA is very accurate in the detection of LN micrometastases of mouse rectal cancer, suggesting that this feasible diagnostic approach is applicable to target sectioning of metastases of resected fresh whole node samples in pathology laboratories. (c) 2009 UICC.

Author information

Author/s: Murayama, Yasutoshi (Y); Harada, Yoshinori (Y); Imaizumi, Katsuichi (K); Dai, Ping (P); Nakano, Keimei (K); Okamoto, Kazuma (K); Otsuji, Eigo (E); Takamatsu, Tetsuro (T);

Affiliation: Department of Pathology and Cell Regulation, Kyoto Prefectural University of Medicine, Kyoto, Japan.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: International journal of cancer. Journal international du cancer (Int J Cancer), published in United States. (Language: eng)

Reference: 2009-Nov; vol 125 (issue 10) : pp 2256-63

Dates: Created 2009/09/28; Completed 2009/11/05;

PMID: 19569177, status: MEDLINE (last retrieval date: 11/5/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article
(including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Aminolevulinic Acid - diagnostic use Animals Female Green Fluorescent Proteins - genetics; metabolism Humans Lymph Nodes - pathology Lymphatic Metastasis

Mice Mice, Inbred BALB C Microscopy, Confocal Photosensitizing Agents - diagnostic use Protoporphyrins - diagnostic use; metabolism Rectal Neoplasms - diagnosis; metabolism Tumor Cells, Cultured

Associated Chemicals: Photosensitizing Agents (0) ; Protoporphyrins (0) ; enhanced green fluorescent protein (0) ; Aminolevulinic Acid (106-60-5) ; Green Fluorescent Proteins (147336-22-9) ; protoporphyrin IX (553-12-8)

Related articles

These are the highest related articles currently in the database:

• An affordable, portable fluorescence imaging device for skin lesion detection using a dual wavelength approach for image contrast enhancement and aminolaevulinic acid-induced protoporphyrin IX. Part I. Design, spectral and spatial characteristics. 30 Dec 2000
• An affordable, portable fluorescence imaging device for skin lesion detection using a dual wavelength approach for image contrast enhancement and aminolaevulinic acid-induced protoporphyrin IX. Part II. In vivo testing. 30 Dec 2000
• Endoscopic fluorescence diagnosis and laser treatment of transitional cell carcinoma of the bladder. 30 Oct 2000
• Increased brain tumor resection using fluorescence image guidance in a preclinical model. 30 Dec 2003
• Comparison of aminolevulinic acid- and hexylester aminolevulinate-induced protoporphyrin IX fluorescence for the detection of ovarian cancer in a rat model. 30 Jul 2007
• [Fluorescent diagnostic methods and superficial cancer of the urinary bladder: current status] 29 Apr 2008
• Fluorescence detection of small gastrointestinal tumours: principles, technique, first clinical experience. 30 Oct 2000
• Kinetics of 5-aminolevulinic acid-induced fluorescence in organ cultures of bronchial epithelium and tumor. 30 Dec 2001
• Photodetection of basal cell carcinoma using methyl 5-aminolaevulinate-induced protoporphyrin IX based on fluorescence image analysis. 22 Apr 2007
• Photodynamic cystoscopy for detection of bladder tumors. 30 May 1999

See 100+ related articles.

Related Article Map

Legend: - FREE Full text Article. - Abstract only. - Title only. More help.

See a large map of 100+ related articles.