Research article summary (published 29 Nov 2009):

Association of HPV16 E6 variants with diagnostic severity in cervical cytology samples of 354 women in a US population.

Full Abstract

It has been suggested that DNA sequence variants of HPV16 contribute to differences in the behavior of individual cervical lesions. To address this question, we have analyzed the association of HPV16 variants with diagnostic severity in 354 HPV16-positive Oklahoman women. HPV16 variant status was determined by PCR amplification and DNA sequencing of the E6 open reading frame. European sequences were identified in 86% of samples and 14% were non-European. Of the 51 non-European cases, 61% were Asian-American, 23% African and 16% were Native American variants. European prototype and related variants were present in comparable numbers (43% each) but the relative proportion of each differed with diagnostic category. In general, the proportion of European variants and non-European variants increased with diagnostic severity while the European prototype decreased. When adjusted for age and race (white, black or Hispanic), the increased risk for carcinoma/severe dysplasia for non-European variants was statistically significant with an odds ratio of 3.8 (1.3-10.7). However, the analogous comparison for the European variants, although also showing increased association with carcinoma/severe dysplasia, did not reach statistical significance (OR = 1.6 (95% CI 0.7-3.6). Overall, HPV16 European sequences (both prototype and related variants), were predominant in Oklahoman women including those with cancers. This suggests that while there appear to be differences among the HPV16-variant categories in risk for progression to invasive cancer, all variant categories are associated with the development of invasive cancer.

Author information

Author/s: Zuna, Rosemary E (RE); Moore, William E (WE); Shanesmith, Rebecca P (RP); Dunn, S Terence (ST); Wang, Sophia S (SS); Schiffman, Mark (M); Blakey, Gregory L (GL); Teel, Travis (T);

Affiliation: Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, OK.

Grants: N02-CP-31102 (Agency:NCI NIH HHS) ; R03-CA117523 (Agency:NCI NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't

Journal: International journal of cancer. Journal international du cancer (Int J Cancer), published in United States. (Language: eng)

Reference: 2009-Dec; vol 125 (issue 11) : pp 2609-13

Dates: Created 2009/10/05; Completed 2009/11/06;

PMID: 19569178, status: MEDLINE (last retrieval date: 11/6/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adolescent Adult Aged Aged, 80 and over Cervical Intraepithelial Neoplasia - virology Cytodiagnosis DNA, Neoplasm - genetics DNA, Viral - genetics Female Genetic Variation Human papillomavirus 16 - isolation & purification Human papillomavirus 18 - genetics; isolation & purification

Human papillomavirus 18 - genetics; isolation & purification Humans Middle Aged Oncogene Proteins, Viral - genetics Papillomavirus Infections - virology Polymerase Chain Reaction Prognosis Repressor Proteins - genetics United States Uterine Cervical Neoplasms - virology Vaginal Smears Young Adult

Associated Chemicals: DNA, Neoplasm (0) ; DNA, Viral (0) ; E6 protein, Human papillomavirus type 16 (0) ; Oncogene Proteins, Viral (0) ; Repressor Proteins (0)

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